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Detection of Human Cells in Xenografts


You want to identify the human cells in a xenograft? You want to know which cells in the tissue are of human origin and which are not?

You need an antibody that stains human cells and that gives no signal in mouse or rat cells. It would be nice if the nuclei would be stained to make it easy to identify the cells in the tissue.

This kind of antibody is within easy reach: Anti-Human Nuclei 1590-NUC from PhosphoSolution for immunohistochemistry and immunofluorescent staining of human cells in xenografts.

The antibody can be used in a 1:100 dilution with PFA or aceton fixed cells. For FFPE tissue the antibody works better at 1:20.

A figure showing staining of human cells with clone 235-1 in presence of murine cells and staining of all nuclei with DAPI can be found here.

Application References of Anti-Human Nuclei, clone 235-1

Significant clinical, neuropathological and behavioural recovery from acute spinal cord trauma by transplantation of a well-defined somatic stem cell
Schira J, Gasis M, Estrada V, Hendricks M, Schmitz C, Trapp T, Kruse F, Kögler G, Wernet P, Hartung HP, Müller HW (2011) Brain : a journal of neurology

Ex vivo generation of a highly potent population of circulating angiogenic cells using a collagen matrix.
Kuraitis D, Hou C, Zhang Y, Vulesevic B, Sofrenovic T, McKee D, Sharif Z, Ruel M, Suuronen EJ (2011) Journal of molecular and cellular cardiology.51:187-97. Epub 2011 May 4.

Siomycin A targets brain tumor stem cells partially through a MELK-mediated pathway.
Nakano I, Joshi K, Visnyei K, Hu B, Watanabe M, Lam D, Wexler E, Saigusa K, Nakamura Y, Laks DR, Mischel PS, Viapiano M, Kornblum HI (2011) Neuro-oncology.13:622-34. Epub 2011 May 9.

Transplantation of umbilical cord mesenchymal stem cells alleviates lupus nephritis in MRL/lpr mice.
Gu Z, Akiyama K, Ma X, Zhang H, Feng X, Yao G, Hou Y, Lu L, Gilkeson GS, Silver RM, Zeng X, Shi S, Sun L (2010) Lupus.19:1502-14. Epub 2010 Jul 20.

Clones of ectopic stem cells in the regeneration of muscle defects in vivo.
Yang R, Chen M, Lee CH, Yoon R, Lal S, Mao JJ (2010) PLoS One.5:e13547.

Dystrophin conferral using human endothelium expressing HLA-E in the nonimmunosuppressive murine model of Duchenne muscular dystrophy.
Cui CH, Miyoshi S, Tsuji H, Makino H, Kanzaki S, Kami D, Terai M, Suzuki H, Umezawa A (2010) Hum Mol Genet

Cell transplantation to arrest early changes in an ush2a animal model.
Lu B, Wang S, Francis PJ, Li T, Gamm DM, Capowski EE, Lund RD (2010) Invest Ophthalmol Vis Sci.51:2269-76. Epub 2009 Dec 3.

Functional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice.
Lian Q, Zhang Y, Zhang J, Zhang HK, Wu X, Zhang Y, Lam FF, Kang S, Xia JC, Lai WH, Au KW, Chow YY, Siu CW, Lee CN, Tse HF (2010) Circulation.121:1113-23. Epub 2010 Feb 22.

Therapeutic effects of human mesenchymal stem cells on traumatic brain injury in rats: secretion of neurotrophic factors and inhibition of apoptosis.
Kim HJ, Lee JH, Kim SH (2010) J Neurotrauma.27:131-8.

Anti-Human Antibody for Xenograft Studies

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