Direct labeling of antibodies can offer significant benefits in techniques such as flow cytometry, ELISA, western blotting and immunohistochemistry.
Download now your Guide to Antibody Labeling and Detection (pdf, 350 kB).
Lightning-Link™ antibody labeling kits use a one-step procedure which means that antibody conjugates can be prepared with ease. Hands-on time is less than 30 seconds, irrespective of scale (microgram to milligram amounts). Separation steps are not required and recovery of antibody is close to 100%.
Without the problems of crossover and/or non-specific binding from secondary reagents it becomes far easier to obtain good quality data. A reduction in the number of incubation and wash steps also makes assays less tedious and saves time. Unfortunately, there has been no simple way of making directly labeled primary antibodies - until now.
Lightning-Link™ uses a one-step procedure which means that conjugates can be prepared with ease. Hands-on time is less than 30 seconds, irrespective of scale. Its easy to label as little as 0.1 mg antibody. Separation steps are not required. Recovery of antibody is close to 100%.
Despite its simplicity, Lightning-Link™ is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled manner, creating high quality stable conjugates.
New: 3 x 10 µg Lightning-Link Labeling Mini-Kits!
The list of labels available in the one-step Lightning-Link format:
Conjugation to Enzymes
Lightning-Link HRP (horseradish peroxidase)
Lightning-Link AP (alkaline phosphatase)
Lightning-Link GOx (glucose oxidase)
Conjugation to Fluorescent Proteins
Lightning-Link APC (allophycocyanin)
Lightning-Link B-PE (B-phycoerythrin)
Lightning-Link R-PE (R-phycoerythrin)
Lightning-Link PerCP (peridinin-chlorophyll-protein)
Conjugation to Biotin, Streptavidin or Avidin
Lightning-Link Biotin Type A (for assays with labelled streptavidin, e. g. Strep-HRP)
Lightning-Link Biotin Type B (for assays with immobilized streptavidin)
Conjugation to Tandem Dyes
Lightning-Link Tandem APC/Cy5.5 (?abs: 633 nm, ?fl: 694 nm)
Lightning-Link Tandem APC/Cy7 (?abs: 633 nm, ?fl: 767 nm)
Lightning-Link Tandem PE/Texas Red (?abs: 488 nm, ?fl: 615 nm)
Lightning-Link Tandem PE/Atto594 (?abs: 488 nm, ?fl: 627 nm)
Lightning-Link Tandem PE/Cy5 (?abs: 488 nm, ?fl: 679 nm)
Lightning-Link Tandem PE/Cy5.5 (?abs: 488 nm, ?fl: 695 nm)
Lightning-Link Tandem PE/Cy7 (?abs: 488 nm, ?fl: 776 nm)
Lightning-Link Tandem PerCP/Cy5.5 (?abs: 482 nm, ?fl: 700 nm)
Conjugation to Fluorescent Dyes
Lightning-Link AMCA (?abs: 346 nm, ?fl: 442 nm)
Lightning-Link Atto390 (?abs: 390 nm, ?fl: 479 nm)
Lightning-Link Atto425 (?abs: 436 nm, ?fl: 484 nm)
Lightning-Link Atto465 (?abs: 453 nm, ?fl: 508 nm)
Lightning-Link Fluorescein (?abs: 485 nm, ?fl: 535 nm) (see also BioSTAIN kits)
Lightning-Link Atto488 (?abs: 501 nm, ?fl: 523 nm)
Lightning-Link Atto532 (?abs: 532 nm, ?fl: 553 nm)
Lightning-Link Rhodamine (?abs: 544 nm, ?fl: 576 nm)
Lightning-Link Cy3 (?abs: 550 nm, ?fl: 570 nm)
Lightning-Link FluoProbes547H (?abs: 557 nm, ?fl: 572 nm)
Lightning-Link Atto565 (?abs: 563 nm, ?fl: 592 nm)
Lightning-Link Cy3.5 (?abs: 581 nm, ?fl: 596 nm)
Lightning-Link Texas Red (?abs: 595 nm, ?fl: 615 nm)
Lightning-Link Atto590 (?abs: 600 nm, ?fl: 627 nm)
Lightning-Link Atto594 (?abs: 601 nm, ?fl: 627 nm)
Lightning-Link FluoProbes594H (?abs: 591 nm, ?fl: 616 nm)
Lightning-Link Atto633 (?abs: 629 nm, ?fl: 657 nm)
Lightning-Link Atto637 (?abs: 630 nm, ?fl: 659 nm)
Lightning-Link Cy5 (?abs: 643 nm, ?fl: 667 nm)
Lightning-Link FluoProbes647H (?abs: 653 nm, ?fl: 675 nm)
Lightning-Link Atto655 (?abs: 663 nm, ?fl: 684 nm)
Lightning-Link Atto680 (?abs: 670 nm, ?fl: 710 nm)
Lightning-Link Cy5.5 (?abs: 643 nm, ?fl: 667 nm)
Lightning-Link FluoProbes682H (?abs: 690 nm, ?fl: 709 nm)
Lightning-Link Atto700 (?abs: 700 nm, ?fl: 725 nm)
Lightning-Link Cy7 (?abs: 747 nm, ?fl: 776 nm)
Lightning-Link FluoProbes752H (?abs: 748 nm, ?fl: 772 nm)
See also: Lightning-Link Rapid Kits, also available for DyLight® dyes.
Overview on Lightning-Link Kits for Conjugation to Fluorescent Dyes:
Antibody Concentration and Clean Up Kit
In order tp label all kind of antibodies the Antibody Concentration and Clean Up Kits are helpful. Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.
The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution.
The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.
861-0005 Antibody Concentration and Clean Up Kit (1 column)
861-0010 Antibody Concentration and Clean Up Kit (3 columns)
Tips for Antibody Purification
Download your Antibody Purification Guide (pdf, 574 kB).
Further technical information is available on the Lightning-Link FAQ page.
Download your two-page Lightning-Link Flyer (pdf, ~1.5MB).
There is a limited number of HRP and R-PE trial kits available (25 µg size). Ask [here] whether you can try one - only shipping costs need to be paid.
Lightning-Link™ conjugation technology is protected by worldwide patent filings.
Rapid Antibody Labeling Kits
Antibody Labeling Kits