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Easy Antibody Conjugation

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Direct labeling of antibodies can offer significant benefits in techniques such as flow cytometry, ELISA, western blotting and immunohistochemistry.


Lightning-Link™ antibody labeling kits use a one-step procedure which means that antibody conjugates can be prepared with ease. Hands-on time is less than 30 seconds, irrespective of scale (microgram to milligram amounts). Separation steps are not required and recovery of antibody is close to 100%.

Without the problems of crossover and/or non-specific binding from secondary reagents it becomes far easier to obtain good quality data. A reduction in the number of incubation and wash steps also makes assays less tedious and saves time. Unfortunately, there has been no simple way of making directly labeled primary antibodies - until now.

Lightning-Link™ uses a one-step procedure which means that conjugates can be prepared with ease. Hands-on time is less than 30 seconds, irrespective of scale. Its easy to label as little as 0.1 mg antibody. Separation steps are not required. Recovery of antibody is close to 100%.

Despite its simplicity, Lightning-Link™ is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled manner, creating high quality stable conjugates.

New: 3 x 10 µg Lightning-Link Labeling Mini-Kits!

The list of labels available in the one-step Lightning-Link format:

 

Conjugation to Enzymes

Lightning-Link HRP (horseradish peroxidase)
Lightning-Link AP (alkaline phosphatase)
Lightning-Link GOx (glucose oxidase)

 

Conjugation to Fluorescent Proteins

Lightning-Link APC (allophycocyanin)
Lightning-Link B-PE (B-phycoerythrin)
Lightning-Link R-PE (R-phycoerythrin)
Lightning-Link PerCP (peridinin-chlorophyll-protein)

 

Conjugation to Biotin, Streptavidin or Avidin

Lightning-Link Biotin Type A (for assays with labelled streptavidin, e. g. Strep-HRP)
Lightning-Link Biotin Type B (for assays with immobilized streptavidin)
Lightning-Link Avidin
Lightning-Link Streptavidin

 

Conjugation to Tandem Dyes

Lightning-Link Tandem APC/Cy5.5 (?abs: 633 nm, ?fl: 694 nm)
Lightning-Link Tandem APC/Cy7 (?abs: 633 nm, ?fl: 767 nm)
Lightning-Link Tandem PE/Texas Red (?abs: 488 nm, ?fl: 615 nm)
Lightning-Link Tandem PE/Atto594 (?abs: 488 nm, ?fl: 627 nm)
Lightning-Link Tandem PE/Cy5 (?abs: 488 nm, ?fl: 679 nm)
Lightning-Link Tandem PE/Cy5.5 (?abs: 488 nm, ?fl: 695 nm)
Lightning-Link Tandem PE/Cy7 (?abs: 488 nm, ?fl: 776 nm)
Lightning-Link Tandem PerCP/Cy5.5 (?abs: 482 nm, ?fl: 700 nm)

 

Conjugation to Fluorescent Dyes

Lightning-Link AMCA (?abs: 346 nm, ?fl: 442 nm)
Lightning-Link Atto390 (?abs: 390 nm, ?fl: 479 nm)
Lightning-Link Atto425 (?abs: 436 nm, ?fl: 484 nm)
Lightning-Link Atto465 (?abs: 453 nm, ?fl: 508 nm)
Lightning-Link Fluorescein (?abs: 485 nm, ?fl: 535 nm) (see also BioSTAIN kits)
Lightning-Link Atto488 (?abs: 501 nm, ?fl: 523 nm)
Lightning-Link Atto532 (?abs: 532 nm, ?fl: 553 nm)
Lightning-Link Rhodamine (?abs: 544 nm, ?fl: 576 nm)
Lightning-Link Cy3 (?abs: 550 nm, ?fl: 570 nm)
Lightning-Link FluoProbes547H (?abs: 557 nm, ?fl: 572 nm)
Lightning-Link Atto565 (?abs: 563 nm, ?fl: 592 nm)
Lightning-Link Cy3.5 (?abs: 581 nm, ?fl: 596 nm)
Lightning-Link Texas Red (?abs: 595 nm, ?fl: 615 nm)
Lightning-Link Atto590 (?abs: 600 nm, ?fl: 627 nm)
Lightning-Link Atto594 (?abs: 601 nm, ?fl: 627 nm)
Lightning-Link FluoProbes594H (?abs: 591 nm, ?fl: 616 nm)
Lightning-Link Atto633 (?abs: 629 nm, ?fl: 657 nm)
Lightning-Link Atto637 (?abs: 630 nm, ?fl: 659 nm)
Lightning-Link Cy5 (?abs: 643 nm, ?fl: 667 nm)
Lightning-Link FluoProbes647H (?abs: 653 nm, ?fl: 675 nm)
Lightning-Link Atto655 (?abs: 663 nm, ?fl: 684 nm)
Lightning-Link Atto680 (?abs: 670 nm, ?fl: 710 nm)
Lightning-Link Cy5.5 (?abs: 643 nm, ?fl: 667 nm)
Lightning-Link FluoProbes682H (?abs: 690 nm, ?fl: 709 nm)
Lightning-Link Atto700 (?abs: 700 nm, ?fl: 725 nm)
Lightning-Link Cy7 (?abs: 747 nm, ?fl: 776 nm)
Lightning-Link FluoProbes752H (?abs: 748 nm, ?fl: 772 nm)



See also: Lightning-Link Rapid Kits, also available for DyLight® dyes.

 

Overview on Lightning-Link Kits for Conjugation to Fluorescent Dyes:

 
Lightning-Link®
Lightning-Link® Rapid
Maximal Absorbance
(nm)
Maximal Emission
(nm)
  AMCA  
346
442
  DyLight® 350  
353
432
  Atto390
390
479
         
  Atto425  
436
484
         
  Atto465  
453
508
  Atto488
501
523
  DyLight® 488   493
518
  Fluorescein
485
535
         
  Atto532  
532
553
  Cyanine Dye 3 (Cy3)
550
570
         
  FluoProbes547H  
557
572
  Rhodamine
544
576
  DyLight® 550  
562
576
  Atto565
563
592
  Cyanine Dye 3.5 (Cy3.5)  
581
596
         
  Texas Red  
595
615
  FluoProbes594  
591
616
  DyLight® 594  
593
618
  Atto590  
600
627
  Atto594
601
627
         
  Atto633
629
657
  DyLight® 633  
638
658
  Atto637  
630
659
  Cyanine Dye 5 (Cy5)
643
667
  DyLight® 650  
652
672
  FluoProbes647H
653
675
  Atto655  
663
684
  Cyanine Dye 5.5 (Cy5.5)  
675
694
  FluoProbes682  
690
709
  Atto680  
670
710
  DyLight® 680  
652
672
  Atto700  
700
725
         
  FluoProbes752  
748
772
  Cyanine Dye 7 (Cy7)  
747
776
  DyLight® 755  
754
778
         

 

Antibody Concentration and Clean Up Kit

In order tp label all kind of antibodies the Antibody Concentration and Clean Up Kits are helpful. Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.

The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution.

The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.

861-0005 Antibody Concentration and Clean Up Kit (1 column)
861-0010 Antibody Concentration and Clean Up Kit (3 columns)

 

Tips for Antibody Purification

Download your Antibody Purification Guide (pdf, 574 kB).

 

Antibody Purification for Labeling Scheme

 

FAQ

Further technical information is available on the Lightning-Link FAQ page.

Download your two-page Lightning-Link Flyer (pdf, ~1.5MB).

 

Trial Kits

There is a limited number of HRP and R-PE trial kits available (25 µg size). Ask [here] whether you can try one - only shipping costs need to be paid.

 

Technical Notes

Lightning-Link™ conjugation technology is protected by worldwide patent filings.

 

Related Products

Rapid Antibody Labeling Kits




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