Expression Systems
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Retroviruses are very efficient tools for delivering genes into dividing cells.
The RetroMax™ Retroviral Expression vector system is based on the pCL vector system and has been designed to maximize recombinant–retrovirus titers in a simple, efficient, and flexible experimental system.
All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal and are derived from safety-modified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation, thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.
The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.
Available Expression Systems
| Cat.No | Description | Unit | | 10054K | Beta-galactosidase quantitative kit. It contains all the reagents necessary for detection of B-galactosidase enzyme in cells and tissues. This kit utilizes CPRG as substrate. | 1 kit | | 10049P | Foxp3, human (full length cDNA) in PCLXSN Retroviral expression vector | 1 µg | | 10042P | pCLNCX Expression Vector | 10 µg | | 10053K | The B-galactosidase staining kit contains all the reagents necessary for detection of B-galactosidase enzyme in cells and tissues. This kit utilizes X-gal as substrate. | 1 kit | | 10040K | The complete RetroMax System includes 4 pCL expression plasmids, 3 packaging plasmids, one reporter plasmid, transfection reagents, and manual. | 1 kit | | 10047P | The pCL-10A1 packaging vector is a part of the RetroMax expression system. Retroviruses obtained by cotransfection with pCL-Eco vector will infect most mammalian cells including hamster cells.Sequence information for this plasmid is not available. | 10 µg | | 10046P | The pCL-Ampho packaging vector is a part of the RetroMax expression system. Retroviruses obtained by cotransfection with pCL-Eco vector will infect most mammalian cells, but not hamster cells. Sequence information for this plasmid is not available. | 10 µg | | 10045P | The pCL-Eco packaging vector is a part of the RetroMax expression system. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.Sequence information for this plasmid is not available. | 10 µg | | 10048P | The pCL-MFG-LacZ reporter vector is a part of the RetroMax expression system. It contains a full-length bacterial b-galactosidase (lacZ) under the control of the murine LTR. Sequence information for this plasmid is not available. | 10 µg | | 10044P | The pCLNDX expresion vector is a part of the RetroMax expression system. The cloned genes are under the control of DHFR promoter. | 10 µg | | 10043P | The pCLNRX expression vector is a part of the RetroMax expression system. The cloned genes are under the control of RSV promoter. | 10 µg | | 10041P | The pCLXSN vector is a part of the RetroMax expression system. | 10 µg | | 10041K | This RetroMax System includes four pCL expression plasmids. | 1 kit |
Products for Purification ...
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