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Nuclear Extraction Kit

The Nuclear Extraction Kit provides a simple and convenient method for the isolation of nuclear and cytoplasmic extracts from mammalian cells and tissue samples.
This procedure is relevant to the monitoring of translocation of cell signaling molecules from cytoplasm to the nucleus. Examples include translocation of NF-κB molecules to the nucleus in TNFa treated cells, and translocation of mitogen-activated protein kinase to the nucleus in growth factor treated cells. The Nuclear Extraction kit can be used in the preparation of purified proteins for use in western blotting, Eletrophoretic Mobility Assays (EMSA) and preparative purification of nuclear proteins.
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