|TLR10 - monoclonal antibody (clone 3C10C5) plus TLR10/NF-kB/SEAPorter™ HEK 293 stable cell line and other research tools for TLR10 analysis and screening.
TLR10 also designated as CD290, remains the only mammalian Toll-like Receptor to which ligands have not yet been characterized. However, there is some solid information regarding TLR10.
TLR10 appears to be closely related to TLR1, especially, and TLR6. Like these two TLRs it is thought that TLR10 also forms a heterodimer with TLR2. It also has been shown to heterodimerize with TLR1 (1,2). Clone 3C1035 is expressed on B cells essentially co-presenting with CD19 and also appears on plasmacytoid Dendritic Cells.
Cell surface flow cytometric analysis of TLR10 on human PBMC using 10 ul (1 ug)
of TLR10 antibody and 1 ug of CD19 antibody (IMG-6321D). IMGENEX’s TLR cell surface flow kit (10099K) was used for this test.
In addition to this new antibody IMGENEX also provides other TLR10 Specific antibodies as well as cell lines stably transfected with TLR10 for screening assays.
TLR10/NF-kB/SEAPorter™ HEK 293 (IML-110) is a stably transfected cell line which expresses full-length human Toll-like Receptor 10 (TLR10) with an N-terminal HA tag and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. TLR10 expression in this cell line has been validated by Western blotting (Fig. 1) and flow cytometry (Fig. 2).
The TLR10/NF-kB/SEAPorter™ HEK 293 cell line can be used for screening of unknown TLR10 agonists.
Fig. 1. Western blot analysis of TLR10 expression in the TLR10/NF-kB/SEAPorter™ HEK 293 cell line using an HA antibody (20 ug total protein/lane). Legend. Cont: Control, NF-kB/SEAPorter™ HEK293 cell line (IMGENEX, IML-101). Fig. 2. Flow analysis of TLR10 expression in the TLR10/NF-kB/SEAPorter™ HEK 293 cell line. (View complete caption)
1. Hasan, U. et al 2005 J Immunology 174:2942-2950
2. Guan, Y. et al 2010 J Immunology 184:5094-5103