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 What area of the brain is apoptotic? Detect neurodegeneration in vivo with FLIVOResearchers can now quantify neuronal cell death in living animals using ICT’s new FLIVO™ fluorescent in vivo apoptosis detection kits. FLIVO™ is an injectable fluorescent probe that crosses the blood-brain barrier and is used to quantitate caspase activity in the brain. It is a direct stain; once labeled, tissues are ready for analysis and no further processing is necessary. FLIVO™ is very easy to use. Just inject it into the animal and let it circulate 30-45 minutes. Dying apoptotic neurons fluoresce green (Figure 2 or 3) or red, and can be counter-stained with other reagents. Detect neurodegeneration in vitro with FLICAICTs FLICA™ kits make it easy to measure cell death and apoptosis in cultured neurons. Just add the reagent to the media, let it incubate 1- 4 hours, wash the cells and read with a fluorescence plate reader, microscope, or flow cytometer. Apoptotic cells fluoresce red or green. Quantify cholinesterase activity in vitroWith ICT’s new cholinesterase assay, researchers now have an easy way to directly quantitate and localize active cholinesterase enzymes in live, intact cells - no lysis or permeabilization steps are required. This unique kit is not an ELISA and does not use antibodies - instead it is based on physostigmine, a known cholinesterase inhibitor, linked to a green fluorescent label, fluorescein (Fl). ICT’s Ph-Fl reagent is cell permeant, so you don’t have to lyse the cells or permeabilize the membranes. Because this kit does not use antibodies, there is no cross-reaction with inactive or pro forms of the enzyme. Only cells with the active cholinesterase enzyme will fluoresce. ICT Product Order Info ... |
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