The addition of a carrier to cytokine solutions can increase the stability and shelf-life of the protein. The use of bovine serum albumin is quite common for this purpose, but has the disadvantage of being of animal origin and quite frequently increases the endotoxin level of the resulting solution. HumanZyme has introduced recombinant Human Serum Albumin expressed in engineered human cells cultured in serum-free chemically defined media. HSAHuXp has ultra low endotoxin levels and is ideal for use as a carrier protein.
Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the protein can be stored at 4°C for short term only, or at -20°C to -80°C in aliquots for long-term purpose. Avoid repeated freeze-thaw cycles.
Use HSAHuXp as carrier protein when reconstituting cytokines to maintain low endotoxin. It is recommended to prepare stock solutions at 0.1% for reconstitution and addition to cytokines in solution.
The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.