This Kit produces high quality dendritic cells.
HumanKine™ G4 Dendritic Cell (DC) Generation Kit has been developed for the growth and generation of human dendritic cells (DCs). Using HumanKine G4 DC medium, human dendritic cells can be produced from peripheral blood mononuclear cells WITHOUT MEDIUM CHANGE and used either as immature DCs or further cultivated into mature DCs.
Protocol in Brief
Reconstitute Cytokine Cocktail
G4 DC Basal Medium
A 10 mL culture requires only 10 mL medium. (plating 3-5 x 105 cells/ml)
A certificate of analysis is available on request.
Storage and Stability
G4 DC Basal Medium: Store at 4ºC, protect from light. Medium is stable for six months from date of production.
Adding the Growth Factor Cocktail to the G4 DC Basal Medium produces G4 Dendritic Cell Generation Medium requiring no medium addition or change during the incubation period.
The resulting cells are monocyte-derived immature DCs that are highly phagocytic with the immature DC surface expression phenotype (CD14neg, CD83neg, CD80low, CD86low, MHC Iint, and MHCint) and can migrate in response to CCR5 agonist (RANTES), but not to CCR7 agonists (ELC/SLC).
G4 Dendritic Cell Generation Kits Order Info ...