HOPE® is the formalin-free fixation technique for molecular pathology and research.
Tissue conservation using the new HOPE®-technique results in paraffin embedded tissue blocks with native structure of the macro-molecules (protein, DNA, RNA) in the tissue conserved.
During HOPE® fixation, tissue samples are incubated in HOPE® I solution, then in HOPE® II solution, followed by a dehydration with acetone and subsequent embedding in paraffin.
For this tissue processing no alcohols or aldehydes (formalin, glutaraldehyde) are used. As a consequence, there is no cross-linking of macro-molecular structures during the process. This opens up new opportunities to characterize tissues out of paraffin blocks.
Many "cryo-type" antibodies are working on HOPE® fixed tissue sections and many antibodies are working without any heat pretreatment or proteolytic digestion steps often necessary for formalin fixed tissue sections.
There is no "over-fixation" with HOPE®, as is known with formalin, when keeping the tissue specimen in the fixation solution. This results in a more standardized fixation process.
RNA, DNA and proteins are conserved in high quality, thus making subsequent applications possible like e.g. PCR, RT-PCR, Northern-Blot, Immunohistochemistry (IHC), in situ hybridization (ISH), Western-Blot (WB) and many others, out of the same tissue blocks.
Here you will find protocols for fixing tisssue with the HOPE®-technique and for subsequent applications when using HOPE®-fixed paraffin embedded tissues.
Complete Instructions for use (German/English) for tissue fixation and embedding using the HOPE®-technique.
What does "HOPE" mean?
HOPE® is the abbreviation for "HEPES-Glutamic Acid Buffer Mediated Organic Solvent Protection Effect". Tissue is protected by a HEPES/glutamic-acid buffer against negative effects of organic fixation solutions (e.g. acetone).
How should HOPE®-tissue blocks be stored?
We recommend storage of HOPE®-tissue blocks at 4°C. This ensures high quality conservation of protein, DNA and RNA for molecular pathology and research applications for many years.
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