Antibodies to Enzymes

Some popular examples:

• Antibodies to Oxidoreductases
• Antibodies to Transferasases e. g.
  • Antibodies to methyltransferases
  • Antibodies to protein tyrosine kinases
  • Antibodies to protein serine/threonine kinases
  • Antibodies to dual specificity protein kinases
• Antibodies to Hydrolases e.g.
  • Antibodies to esterases like nucleases, phosphodiesterase, lipases, phosphatases
  • Antibodies to glycosylases
  • Antibodies to proteases and peptidases
• Antibodies to Lyases
• Antibodies to Isomerases e. g.
  • Antibodies to cis-trans isomerases
• Antibodies to Ligases e. g.
  • Antibodies to carbon nitrogen bond ligases like E2 and E3 ubiquitin-conjugating enzymes

A more general approach:

EC 1. Oxidoreductases

• EC 1.1 Acting on the CH-OH group of donors
  • EC 1.1.1 With NAD or NADP as acceptor
  • EC 1.1.2 With a cytochrome as acceptor
  • EC 1.1.3 With oxygen as acceptor
  • EC 1.1.4 With a disulfide as acceptor
  • EC 1.1.5 With a quinone or similar compound as acceptor
  • EC 1.1.99 With other acceptors
• EC 1.2 Acting on the aldehyde or oxo group of donors
  • EC 1.2.1 With NAD or NADP as acceptor
  • EC 1.2.2 With a cytochrome as acceptor
  • EC 1.2.3 With oxygen as acceptor
  • EC 1.2.4 With a disulfide as acceptor
  • EC 1.2.7 With an iron-sulfur protein as acceptor
  • EC 1.2.99 With other acceptors
• EC 1.3 Acting on the CH-CH group of donors
  • EC 1.3.1 With NAD or NADP as acceptor
  • EC 1.3.2 With a cytochrome as acceptor
  • EC 1.3.3 With oxygen as acceptor
  • EC 1.3.5 With a quinone or related compound as acceptor
  • EC 1.3.7 With an iron-sulfur protein as acceptor
  • EC 1.3.99 With other acceptors
• EC 1.4 Acting on the CH-NH2 group of donors
  • EC 1.4.1 With NAD or NADP as acceptor
  • EC 1.4.2 With a cytochrome as acceptor
  • EC 1.4.3 With oxygen as acceptor
  • EC 1.4.4 With a disulfide as acceptor
  • EC 1.4.7 With an iron-sulfur protein as acceptor
  • EC 1.4.99 With other acceptors
• EC 1.5 Acting on the CH-NH group of donors
  • EC 1.5.1 With NAD or NADP as acceptor
  • EC 1.5.3 With oxygen as acceptor
  • EC 1.5.4 With a disulfide as acceptor
  • EC 1.5.5 With a quinone or similar compound as acceptor
  • EC 1.5.7 With an iron-sulfur protein as acceptor
  • EC 1.5.8 With a flavin as acceptor
  • EC 1.5.99 With other acceptors
• EC 1.6 Acting on NADH or NADPH
  • EC 1.6.1 With NAD or NADP as acceptor
  • EC 1.6.2 With a heme protein as acceptor
  • EC 1.6.3 With oxygen as acceptor
  • EC 1.6.4 With a disulfide as acceptor
  • EC 1.6.5 With a quinone or similar compound as acceptor
  • EC 1.6.6 With a nitrogenous group as acceptor
  • EC 1.6.7 With an iron-sulfur protein as acceptor
  • EC 1.6.8 With a flavin as acceptor
  • EC 1.6.99 With other acceptors
• EC 1.7 Acting on other nitrogenous compounds as donors
  • EC 1.7.1 With NAD+ or NADP+ as acceptor
  • EC 1.7.2 With a cytochrome as acceptor
  • EC 1.7.3 With oxygen as acceptor
  • EC 1.7.7 With an iron-sulfur protein as acceptor
  • EC 1.7.99 With other acceptors
• EC 1.8 Acting on a sulfur group of donors
  • EC 1.8.1 With NAD or NADP as acceptor
  • EC 1.8.2 With a cytochrome as acceptor
  • EC 1.8.3 With oxygen as acceptor
  • EC 1.8.4 With a disulfide as acceptor
  • EC 1.8.5 With a quinone or similar compound as acceptor
  • EC 1.8.6 With a nitrogenous group as acceptor
  • EC 1.8.7 With an iron-sulfur protein as acceptor
  • EC 1.8.98 With other, known, acceptors
  • EC 1.8.99 With other acceptors
• EC 1.9 Acting on a heme group of donors
  • EC 1.9.3 With oxygen as acceptor
  • EC 1.9.6 With a nitrogenous group as acceptor
  • EC 1.9.99 With other acceptors
• EC 1.10 Acting on diphenols and related substances as donors
  • EC 1.10.1 With NAD or NADP as acceptor
  • EC 1.10.2 With a cytochrome as acceptor
  • EC 1.10.3 With oxygen as acceptor
  • EC 1.10.99 With other acceptors
• EC 1.11 Acting on a peroxide as acceptor
  • EC 1.11.1 Peroxidases
• EC 1.12 Acting on hydrogen as donor
  • EC 1.12.1 With NAD or NADP as acceptor
  • EC 1.12.2 With a cytochrome as acceptor
  • EC 1.12.5 With a quinone or similar compound as acceptor
  • EC 1.12.7 With an iron-sulfur protein as acceptor
  • EC 1.12.98 With other known acceptors
  • EC 1.12.99 With other acceptors
• EC 1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
  • EC 1.13.1 (transferred to EC 1.13.11)
  • EC 1.13.11 With incorporation of two atoms of oxygen
  • EC 1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)
  • EC 1.13.99 Miscellaneous
• EC 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
  • EC 1.14.1 With NADH or NADPH as one donor (deleted sub-subclass)
  • EC 1.14.2 With ascorbate as one donor (deleted sub-subclass)
  • EC 1.14.3 With reduced pteridine as one donor (deleted sub-subclass)
  • EC 1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
  • EC 1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into one donor
  • EC 1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen
  • EC 1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
  • EC 1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen
  • EC 1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen
  • EC 1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen
  • EC 1.14.18 With another compound as one donor, and incorporation of one atom of oxygen
  • EC 1.14.19 With oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
  • EC 1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated
  • EC 1.14.21 With NADH or NADPH as one donor, and the other dehydrogenated
  • EC 1.14.99 Miscellaneous
• EC 1.15 Acting on superoxide as acceptor
  • EC 1.15.1
• EC 1.16 Oxidizing metal ions
  • EC 1.16.1 With NAD or NADP as acceptor
  • EC 1.16.3 With oxygen as acceptor
  • EC 1.16.8 With flavin as acceptor
• EC 1.17 Acting on CH or CH2 groups
  • EC 1.17.1 With NAD or NADP as acceptor
  • EC 1.17.3 With oxygen as acceptor
  • EC 1.17.4 With a disulfide as acceptor
  • EC 1.17.5 With a quinone or similar compound as acceptor
  • EC 1.17.99 With other acceptors
• EC 1.18 Acting on iron-sulfur proteins as donors
  • EC 1.18.1 With NAD+ or NADP+ as acceptor
  • EC 1.18.2 With dinitrogen as acceptor (transferred to EC 1.18.6)
  • EC 1.18.3 With H+ as acceptor
  • EC 1.18.6 With dinitrogen as acceptor
  • EC 1.18.96 With other, known, acceptors
  • EC 1.18.99 With H+ as acceptor
• EC 1.19 Acting on reduced flavodoxin as donor
  • EC 1.19.6 With dinitrogen as acceptor
• EC 1.20 Acting on phosphorus or arsenic in donors
  • EC 1.20.1 Acting on phosphorus or arsenic in donors, with NAD(P)+ as acceptor
  • EC 1.20.4 Acting on phosphorus or arsenic in donors, with disulfide as acceptor
  • EC 1.20.98 Acting on phosphorus or arsenic in donors, with other, known acceptors
  • EC 1.20.99 Acting on phosphorus or arsenic in donors, with other acceptors
• EC 1.21 Acting on X-H and Y-H to form an X-Y bond
  • EC 1.21.3 With oxygen as acceptor
  • EC 1.21.4 With a disulfide as acceptor
  • EC 1.21.99 With other acceptors
• EC 1.97 Other oxidoreductases
  • EC 1.97.1
• EC 1.98 Enzymes using H2 as reductant
  • EC 1.98.1
• EC 1.99 Other enzymes using O2 as oxidant
  • EC 1.99.1 Hydroxylases (now covered by EC 1.14)
  • EC 1.99.2 Oxygenases (now covered by EC 1.13)

EC 2. Transferases

• EC 2.1: Transferring One-Carbon Groups
  • EC 2.1.1: Methyltransferases
  • EC 2.1.2: Hydroxymethyl-, Formyl- and Related Transferases
  • EC 2.1.3: Carboxy- and Carbamoyltransferases
  • EC 2.1.4: Amidinotransferases
• EC 2.2: Transferring Aldehyde or Ketonic Groups
  • EC 2.2.1: Transketolases and Transaldolases
• EC 2.3: Acyltransferases
  • EC 2.3.1: Transferring groups other than amino-acyl groups
  • EC 2.3.2: Aminoacyltransferases
  • EC 2.3.3: Acyl groups converted into alkyl on transfer
• EC 2.4: Glycosyltransferases
  • EC 2.4.1: Hexosyltransferases
  • EC 2.4.2: Pentosyltransferases
  • EC 2.4.99: Transferring Other Glycosyl Groups
• EC 2.5: Transferring Alkyl or Aryl Groups, Other than Methyl Groups
• EC 2.6: Transferring Nitrogenous Groups
  • EC 2.6.1: Transaminases
  • EC 2.6.2: Amidinotransferases
  • EC 2.6.3: Oximinotransferases
  • EC 2.6.99: Transferring Other Nitrogenous Groups
• EC 2.7: Transferring Phosphorus-Containing Groups
  • EC 2.7.1: Phosphotransferases with an alcohol group as acceptor
  • EC 2.7.2: Phosphotransferases with a carboxy group as acceptor
  • EC 2.7.3: Phosphotransferases with a nitrogenous group as acceptor
  • EC 2.7.4: Phosphotransferases with a phosphate group as acceptor
  • EC 2.7.5: Phosphotransferases with regeneration of donors, apparently catalysing intramolecular transfers
  • EC 2.7.6: Diphosphotransferases
  • EC 2.7.7: Nucleotidyltransferases
  • EC 2.7.8: Transferases for other substituted phosphate groups
  • EC 2.7.9: Phosphotransferases with paired acceptors (dikinases)
  • EC 2.7.10: Protein-tyrosine kinases
  • EC 2.7.11: Protein-serine/threonine kinases
  • EC 2.7.12: Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
  • EC 2.7.13: Protein-histidine kinases
  • EC 2.7.99: Other protein kinases
• EC 2.8: Transferring Sulfur-Containing Groups
  • EC 2.8.1: Sulfurtransferases
  • EC 2.8.2: Sulfotransferases
  • EC 2.8.3: CoA-transferases
  • EC 2.8.4: Transferring alkylthio groups
• EC 2.9: Transferring Selenium-Containing Groups
  • EC 2.9.1: Selenotransferases

EC 3. Hydrolases

• EC 3.1: Acting on Ester Bonds
  • EC 3.1.1: Carboxylic Ester Hydrolases
  • EC 3.1.2: Thioester Hydrolases
  • EC 3.1.3: Phosphoric Monoester Hydrolases
  • EC 3.1.4: Phosphoric Diester Hydrolases
  • EC 3.1.5: Triphosphoric Monoester Hydrolases
  • EC 3.1.6: Sulfuric Ester Hydrolases
  • EC 3.1.7: Diphosphoric Monoester Hydrolases
  • EC 3.1.8: Phosphoric Triester Hydrolases
  • EC 3.1.11: Exodeoxyribonucleases Producing 5-Phosphomonoesters
  • EC 3.1.13: Exoribonucleases Producing 5-Phosphomonoesters
  • EC 3.1.14: Exoribonucleases Producing 3-Phosphomonoesters
  • EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5-Phosphomonoesters
  • EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3-Phosphomonoesters
  • EC 3.1.21: Endodeoxyribonucleases Producing 5-Phosphomonoesters
  • EC 3.1.22: Endodeoxyribonucleases Producing 3-Phosphomonoesters
  • EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5
  • EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases
  • EC 3.1.26: Endoribonucleases Producing 5-Phosphomonoesters
  • EC 3.1.27: Endoribonucleases Producing 3-Phosphomonoesters
  • EC 3.1.30: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5-Phosphomonoesters
  • EC 3.1.31: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3-Phosphomonoesters
• EC 3.2: Glycosylases
  • EC 3.2.1: Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds
  • EC 3.2.2: Hydrolysing N-Glycosyl Compounds
  • EC 3.2.3: Hydrolysing S-Glycosyl Compounds
• EC 3.3: Acting on Ether Bonds
  • EC 3.3.1: Thioether and trialkylsulfonium hydrolases
  • EC 3.3.2: Ether Hydrolases
• EC 3.4: act on peptide bonds - Peptidase
  • EC 3.4.1 ?-amino acyl peptide hydrolases (discontinued)
  • EC 3.4.2 Peptidyl amino acid hydrolases (discontinued)
  • EC 3.4.3 Dipeptide Hydrolases (discontinued)
  • EC 3.4.4 Peptidyl Peptide Hydrolases (discontinued)
  • EC 3.4.11 Aminopeptidases
  • EC 3.4.13 Dipeptidases
  • EC 3.4.14 Dipeptidyl peptidases and tripeptidyl peptidases
  • EC 3.4.15 Peptidyl dipeptidases
  • EC 3.4.16 Serine type carboxypeptidases
  • EC 3.4.17 Metallocarboxypeptidases
  • EC 3.4.18 Cysteine type carboxypeptidases
  • EC 3.4.19 Omega peptidases
  • EC 3.4.21: Serine proteases
  • EC 3.4.22 Cysteine proteases
  • EC 3.4.23 Aspartic endopeptidases
  • EC 3.4.24: Metallopeptidases
  • EC 3.4.25 Threonine endopeptidases
  • EC 3.4.99 Endopeptidases of unknown catalytic mechanism
• EC 3.5: act on carbon-nitrogen bonds, other than peptide bonds
  • EC 3.5.1: linear amides
  • EC 3.5.2: cyclic amides
  • EC 3.5.3: linear amidines
  • EC 3.5.4: cyclic amidines
  • EC 3.5.5: nitriles
  • EC 3.5.99: other compounds
• EC 3.6: act on acid anhydrides
  • EC 3.6.1
  • EC 3.6.2
  • EC 3.6.3
  • EC 3.6.4
  • EC 3.6.5
• EC 3.7: act on carbon-carbon bonds
• EC 3.8: act on halide bonds
• EC 3.9: act on phosphorus-nitrogen bonds
• EC 3.10: act on sulfur-nitrogen bonds
• EC 3.11: act on carbon-phosphorus bonds
• EC 3.12: act on sulfur-sulfur bonds
• EC 3.13: act on carbon-sulfur bonds

EC 4. Lyases

• EC 4.1: Carbon-Carbon Lyases
  • EC 4.1.1: Carboxy-Lyases
  • EC 4.1.2: Aldehyde-Lyases
  • EC 4.1.3: Oxo-Acid-Lyases
  • EC 4.1.99: Other Carbon-Carbon Lyases
• EC 4.2: Carbon-Oxygen Lyases
  • EC 4.2.1: Hydro-Lyases
  • EC 4.2.2: Acting on Polysaccharides
  • EC 4.2.3: Acting on phosphates
  • EC 4.2.99: Other Carbon-Oxygen Lyases
• EC 4.3: Carbon-Nitrogen Lyases
  • EC 4.3.1: Ammonia-Lyases
  • EC 4.3.2: Amidine-Lyases
  • EC 4.3.3: Amine-Lyases
  • EC 4.3.99: Other Carbon-Nitrogen Lyases
• EC 4.4: Carbon-Sulfur Lyases
• EC 4.5: Carbon-Halide Lyases
• EC 4.6: Phosphorus-Oxygen Lyases
• EC 4.99: Other Lyases

EC 5. Isomerases

• EC 5.1: Epimerases and racemases
  • EC 5.1.1: Acting on Amino acids and Derivatives
  • EC 5.1.2: Acting on Hydroxy acids and Derivatives
  • EC 5.1.3: Acting on Carbohydrates and Derivatives
  • EC 5.1.99: Acting on Other Compounds
• EC 5.2: cis-trans-Isomerases
• EC 5.3: Intramolecular Oxidoreductases
  • EC 5.3.1: Interconverting Aldoses and Ketoses
  • EC 5.3.2: Interconverting Keto- and Enol-Groups
  • EC 5.3.3: Transposing C=C Bonds
  • EC 5.3.4: Transposing S-S Bonds
  • EC 5.3.99: Other Intramolecular Oxidoreductases
• EC 5.4: Intramolecular Transferases
  • EC 5.4.1: Transferring Acyl Groups
  • EC 5.4.2: Phosphotransferases (Phosphomutases)
  • EC 5.4.3: Transferring Amino Groups
  • EC 5.4.4: Transferring hydroxy groups
  • EC 5.4.99: Transferring Other Groups
• EC 5.5: Intramolecular Lyases
• EC 5.99: Other Isomerases

EC 6. Ligases

• EC 6.1: Forming Carbon-Oxygen Bonds
  • EC 6.1.1: Ligases Forming Aminoacyl-tRNA and Related Compounds (Aminoacyl tRNA synthetase)
• EC 6.2: Forming Carbon-Sulfur Bonds
  • EC 6.2.1: Acid-Thiol Ligases
• EC 6.3: Forming Carbon-Nitrogen Bonds
  • EC 6.3.1: Acid-Ammonia (or Amine) Ligases (Amide Synthases)
  • EC 6.3.2: Acid-Amino-Acid Ligases (Peptide Synthases)
  • EC 6.3.3: Cyclo-Ligases
  • EC 6.3.4: Other Carbon-Nitrogen Ligases
  • EC 6.3.5: Carbon-Nitrogen Ligases with Glutamine as Amido-N-Donor
• EC 6.4: Forming Carbon-Carbon Bonds
• EC 6.5: Forming Phosphoric Ester Bonds
• EC 6.6: Forming Nitrogen-Metal Bonds
  • EC 6.6.1: Forming Coordination Complexes