Hybrid-R for the Isolation of Total RNA

Hybrid-R™ for purification of up to 500 µg total RNA from cells, tissues, plant, E. coli and various biological samples.

Fast procedure delivering high-quality total RNA in 30 minutes
Ready-to-use RNA for high performance in any downstream application like RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assays
No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
High purity
No genomic DNA contamination
Sample size: ~100 mg tissue or 1x10^7 cells
Yield: 500 µg
Kit size: 100 preparations
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Hybrid-R™ provides an easy and rapid method for the isolation of highly purified total RNA from samples of human, animal, plant, yeast, bacterial and viral origin. Hybrid-R eliminates alcohol precipitation by binding of RNA with column, allowing rapid and convenient preparation from a large number of samples simultaneously.

Hybrid-R™ can yield up to 500 µg depending on the type of tissue sample used and complete all process to prepare total RNA in just 30 minutes. The purified total RNA is suitable for the isolation of mRNA, northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RNase protection assays and other analytical procedures.
Manual (pdf)