Akt1, active, Recombinant, Human (Rac PKa, PKBa)

Akt1, active, Recombinant, Human (Rac PKa, PKBa)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
A1125-01L.10 10 µg - -

3 - 19 business days*

985.00€
 
Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST... more
Product information "Akt1, active, Recombinant, Human (Rac PKa, PKBa)"
Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST tag, co-expressed with PDK1 in an Baculovirus infected Sf9 cell expression system. Specific Activity: ~12pmol/min/ug , Assay Conditons: Enzyme reaction is conducted in a buffer , containing 50mM HEPES, pH 7.5, 10mM , magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, and 2uM substrate (SER/THR 6) at RT for 1 hour. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Keywords: PKB alpha, EC=2.7.11.1, RAC-PK-alpha, Protein kinase B, Proto-oncogene c-Akt, Protein kinase B alpha, RAC-alpha serine/threonine-protein kinase
Supplier: United States Biological
Supplier-Nr: A1125-01L

Properties

Application: Enzyme assays
Conjugate: No
Format: Highly Purified

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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