JMJD3 chemiluminescent assay kit

JMJD3 chemiluminescent assay kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
BPS-50406 96 rxns - -

3 - 15 business days*

1,065.00€
 
The JMJD3 Chemiluminescent Assay Kit is designed to measure JMJD3 activity for screening and... more
Product information "JMJD3 chemiluminescent assay kit"
The JMJD3 Chemiluminescent Assay Kit is designed to measure JMJD3 activity for screening and profiling applications. JMJD3 is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3. The JMJD3 Assay Kit comes in a convenient format, with 8-well strips precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JMJD3 for 100 enzyme reactions. The key to the JMJD3 Direct Activity Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Note: The kit is also suitable for use with cell extracts, but non-specific demethylase activity may be detected. Components: JMJD3-4 µg, primary antibody 6-25 µl, secondary HRP-labeled antibody 2- 10 µL, 4x JMJD3 assay buffer 3 ml, blocking buffer 50 ml, HRP chemiluminescent substrate 6 ml ea (2 components), 12 x 8-well strips
Keywords: JMJD3, KDM6B,
Supplier: BPS Bioscience
Supplier-Nr: 50406

Properties

Application: Enzyme kinetics, HTS
Format: Homog.

Handling & Safety

Storage: vT
Shipping: -80°C (International: -80°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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