|Detection of Human and Mouse RelA/p65 by Western Blot (human & mouse) and Immunoprecipitation (human). |
Samples: Whole cell lysate from HeLa (H; 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells.
Antibodies: Affinity purified rabbit anti-RelA/p65 antibody A301-824A used for Western Blot at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for Immunoprecipitation at 3 mcg/mg lysate. RelA/p65 was also immunoprecipitated by rabbit anti-RelA/p65 antibody A301-823A, which recognizes an upstream epitope. For blotting immunoprecipitated RelA/p65, the ReliaBLOT® Reagents and Procedures (Cat. No. WB120) were used.
Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
|Detection of Human NF-kappaB1 by Western Blot and Immunoprecipitation. |
Samples: Whole cell lysate from HeLa (H; 50 mcg) and Jurkat (J; 50 mcg for Western Blot; 1 mg for IP, 20% of IP loaded) cells.
Antibodies: Affinity purified rabbit anti-NF-kappaB1 antibody A301-820A used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NF-kappaB1 was also immunoprecipitated by rabbit anti-NF-kappaB1 antibody A301-819A, which recognizes an upstream epitope. For blotting immunoprecipitated NF-kappaB1, the ReliaBLOT® Reagents and Procedures (Cat. No. WB120) were used.
Detection: Chemiluminescence with exposure times of 1 minute (A) and 3 seconds (B).
|I kappa B-beta||A301-828A||A301-828A|
| A301-819A |
|NF-kappaB2|| A301-821A |
| A301-821A |
| A301-823A |
| A301-825A |
|CKII alpha|| A300-196A |
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