Top 20 Bethyl Polyclonal Antibodies
What are the most interesting antibodies from Bethyl?
This question is hard to answer. Bethyl offers more than 2500 antibodies. It depends on your research interest. Nevertheless Biomol has chosen some interesting antibodies for this introduction of Bethyl polyclonal antibodies:
for WB and ICC)
53BP1 (p53 Binding Protein 1) is an early participant in the cellular response to DNA double-strand breaks. 53BP1 functions in a redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage. Alternate names for 53BP1 include Tumor protein p53 binding protein 1 and p202.
for WB and IP)
Cytoskeletal beta-actin is one of six highly conserved actin isoforms that is expressed ubiquitously in eukaryotic cells. Cytoskeletal beta-actin functions to maintain cell structure and plays a role in motility. The antibody can be used for the loading control in western blotting.
Chk1 functions as a checkpoint protein kinase that is critical to the regulation of the Cdc25a and Cdc25c phosphatases that promote S and G2/M phase progression, respectively. Chk1 kinase activity inhibits cell cycle progression in the presence of DNA damage by phosphorylating Cdc25a and Cdc25c and causing the degradation of Cdc25a and cytoplasmic sequestration of Cdc25c. Alternate designations for Chk1 include serine/threonine-protein kinase Chk1, and CHEK1.
for WB and IP)
HdmX/MDM4 is a RING-finger domain containing protein that interacts with p53. HdmX/MDM4 may influence p53 stability through an interaction with the structurally similar MDM2, an ubiquitin-ligase that targets p53 for proteasomal degradation. HdmX/MDM4 may influence p53 by directly attenuating p53 transcriptional activity. Alternate names for HdmX/MDM4 include p53-binding protein mdm4, mdm2-like p53-binding protein, double minute 4 protein, MDMX, MRP1, MGC132766, and DKFZp781B1423.
for WB and IP)
Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme that catalyzes the conversion of glucose-6-phosphate into 6-phosphogluconolactone and maintains the levels of the electron donor, NADPH. G6PD is an x-linked housekeeping gene whose deficiency can result in neonatal jaundice, acute hemolysis, or chronic non-spherocytic hemolytic anemia.
for WB and IHC)
gdf8 (growth and differentiation factor 8), also known as myostatin, is a member of the TGF-beta family of growth factors and functions as a negative regulator of muscle growth. Loss-of-function mutations in gdf8 have been shown to result in increased skeletal muscle mass in mice, cattle, and humans. Alternate names for gdf8 include GDF-8, MSTN, and GDF8.
for WB and IF)
H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage. Alternate names for H2AX include histone H2A.x, and H2a/x.
Hypoxia-inducible factor-1 (HIF1) is a basic helix-loop-helix (bHLH)-PER-ARNT-sim (PAS) transcription factor that plays a role in the cellular response to hypoxia. HIF1 is a heterodimer of an HIF1-alpha and HIF1-beta subunit. During normoxia, HIF-1alpha is regulated by ubiquitination and interaction with the von-Hippel-Lindau tumor suppressor protein (pVHL). Alternate names for HIF1-alpha include HIF-1 alpha, ARNT-interacting protein, member of PAS protein 1, basic-helix-loop-helix-PAS protein MOP1, HIF1A, MOP1, and PASD8.
for WB, EMSA, ChIP, and IHC)
MafA is a member of the Maf basic leucine-zipper containing transcription factor family. MafA is an islet-enriched protein that plays a role in beta cell-specific and glucose-responsive insulin gene transcription. MafA is expressed exclusively in cell populations that will become islet beta cells. Alternate names for MafA include v-maf musculoaponeurotic fibrosarcoma oncogene homolog A, transcription factor MafA, pancreatic beta-cell-specific transcriptional activator, hMafA, and RIPE3b1.
for WB, IP, IF, ChIP, and IHC)
Menin is the gene product of the putative tumor suppressor MEN1 associated with the syndrome termed multiple endocrine neoplasia type 1 that may function in the regulation of DNA synthesis or the response to DNA damage. Menin has been shown to interact with JunD, a member of the AP-1 heterodimer and inhibit transcription. Menin has also been shown to interact with Pem, FANCD2, RPA, NMMHCII-A, GFAP, vimentin, Hsp70, NfkappaB proteins, Smads, mSin3A and is part of the MLL2 and MLL1 Set1-like histone H3 K4 methyltransferase complex where it interacts directly with ASH2, Rbbp5, and WDR5. As part of the MLL complexes, menin may mediate its tumor suppressor activity via the regulation of histone methylation of HOX and CDK inhibitor genes. Alternate names for Menin are MEAI, multiple endocrine adenomatosis 1, suppressor Candidate Gene 2, and SCG2.
for WB, IP, and IHC)
MLL1 (mixed lineage leukemia 1) has been found to participate in a multitude of chromosomal translocations that are associated with a variety of hematologic malignancies. MLL1 functions as a transcriptional regulator and plays and important role in HOX gene expression during embryonic development. Alternate names for MLL1 include zinc-finger protein HRX, ALL-1, trithorax-like protein, MLL, ALL1, HRX, HTRX1, THRX, TRX1, CXXC7, MLL1A, FLJ11783, and MLL/GAS7.
for WB and ICC)
Mutations in NBS1 (Nijmegen Breakage Syndrome 1), also known as nibrin, are associated with the autosomal recessive syndrome, Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. At the cellular level, NBS1 is part of the MRE11/RAD50 double-strand break repair complex that is critical to the DNA damage response, DNA recombination, telomere integrity, and cell cycle checkpoint control. Alternate names for NBS1 include cell cycle regulatory protein p95, NBS, NBN, ATV, AT-V1, AT-V2, FLJ10155, and MGC87362.
PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar. PBEF is also known as PBEF1, Pre-B cell Enhancing Factor 1, NAMPT, and nicotinamide phosphoribosyltransferase.
PHD1 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity. Alternate names for PHD1 include PH1, PHD1, prolyl hydroxylase domain-containing protein 1, HIFPH1, HIF-prolyl hydroxylase 1, EGLN2, EGL nine homolog 2, EIT6, estrogen-induced tag 6, and DKFZp434E026.
PHD2 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity. Alternate names for PHD2 include PHD2, PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, EGLN1, egl nine homolog 1, and C1orf12.
PHD3 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity. Alternate names for PHD3 include PH3, PHD3, Prolyl hydroxylase domain-containing 3, EGLN3, egl nine homolog 3, SM-20, and FLJ21620.
Raptor (regulatory-associated protein of mTOR) is part of the rapamycin sensitive mTORC1 complex which includes mTOR, and mLst8/GbetaL. S6K and 4EBP1 are substrates of mTORC1 kinase activity. Raptor is required for mTOR’s ability to phosphorylate 4E-BP1 and physically interacts with 4EBP1 to act as a potential scaffold that bridges mTOR and 4EBP1 or S6K. The mTORC1 complex integrates cell signals to promote cell growth in response to growth factors. Raptor is also known as P150 target of rapamycin (TOR)-scaffold protein, and KIAA1303.
for WB and IP and A300-458A
for WB and IP)
Rictor (rapamycin insensitive companion of TOR) is part of the rapamycin insensitive mTORC2 complex which includes mTOR, mLST8/GbetaL, Sin1, and protor 1. The mTORC2 complex appears to function in the regulation of cytoskeletal organization and has been shown to phosphorylate Akt/PKB. Rictor is also known as mAVO3, KIAA1999, MGC39830, and DKFZp686B11164.
for WB and IP)
Topoisomerases regulate topological states of DNA. DNA topoisomerase II-binding protein 1 (TopBP1) is a protein that interacts with the C-terminal region of topoisomerase II beta (Topo II beta). TopBP1 function has been found to be important to various aspects of cellular growth control such as DNA replication, the DNA damage checkpoint, and cell survival. TopBP1 interacts with several proteins through its BRCA1 C-terminal (BRCT) motifs. TopBP1 interacts and inhibits E2F1 transcriptional activity through an Rb-independent mechanism that involves recruitment of the SWI/SNF chromatin-remodeling complex proteins Brg1 and Brm. TopBP1 is also known as DNA topoisomerase 2-binding protein, DNA topoisomerase II beta-binding protein 1, TOPBP1, and KIAA0259.
Wiskott-Aldrich syndrome protein WASP is part of the WAS family of proteins that mediate cell signals that regulate the dynamics of the actin cytoskeleton. WASP associates with Cdc42, a Rho-family GTPase, and the cytoskeletal organizing complex, Arp2/3, to stimulate actin-polymerization. WASP also binds with RAC, NCK, FYN, SRC kinase FGR, BTK, ABL, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. WASP is also known as WAS, Wiskott - Aldrich syndrome (eczema-thrombocytopenia) Protein, THC, Thrombocytopenia 1 (X-linked), IMD2.
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Complete Listing of Bethyl's Polyclonal Antibodies