Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders (1). In one study, the predominant responses to histones in SLE sera were...

Applications: , Suitable for use in Western Blot and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 dilution of this lot detected acetyl-Histone H4 (Lys5) in acid extracts from sodium butyrate treated HeLa cells , ChIP: An independent laboratory has shown that this antibody...

Applications:, Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:500, ELISA: 1:1000, Western Blot: 1:500-1:1000, Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml, Optimal dilutions to...

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker...

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is...