|TEMPase Hot Start Master Mix is a ready-to-use 2.0X master mix.
Simply add primers, template and water to successfully carry out primer extensions and other molecular biology applications.
Ampliqon TEMPase Hot Start DNA Polymerase, the balanced K/NH4+ buffer system, dNTPs and magnesium chloride are present in TEMPase Hot Start Master Mix with TEMPase Buffer II. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 µL.
TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.
TEMPase Hot Start Master Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
€ The choice for convenient use
€ TEMPase Hot Start DNA Polymerase for increased specificity.
€ Ideal for rich amplifications
€ Designed to diminish the formation of a non-specific product.
€ Successful for multiplex reactions.