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| TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of ther-mal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. Once the reaction reaches optimal annealing temperatures, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active TEMPase DNA Polymerase into the reaction. Sensitivity improves multiplex PCR, an applied PCR technique that amplifies several specific targets simultaneously. Applications that previously required two or more reactions can be performed in a single reaction tube. Hence, multiplexing represents a substantial savings of time and costly reagents. Unit Definition One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions. 10x TEMPase Buffer II This is a new optimized buffer system with a balanced Ammonium/Potassium concentration with 15 mM MgCl2. This buffer improves more complicated PCR systems such as multiplex PCR. Key Features €TEMPase Hot Start enzyme for in-creased specificity and product yield €Successful multiplex reactions save time and reagents €Designed to diminish the formation of non-specific product €Detection of low target copy number All Kits Contain TEMPase Hot StartDNA Polymerase, 10X TEMPase Buffer II Tween Free and MgCl2 (25mM). |
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