Quest™ Fluo-8 Calcium Assay Kit for Cellular Analysis

Ideal as calcium assay, brighter than Fluo-3 and Fluo-4.
Utilizing the brightest and most sensitive fluorescent Ca2+ indicator, Screen Quest(TM) Fluo-8 Calcium Assay Kit provides much higher signal intensity and very large fluorescence intensity increase in response to Ca2+ binding. AAT Bioquest’s homogeneous fluorescence-based assay kit detects calcium mobilization across a broad spectrum of biological targets. Easy-to-use protocol does not require a wash step or exogenous addition of a quencher dye, which could adversely affect receptor-ligand interaction kinetics.

Other widely used calcium ion indicators, such as Fluo-3, Fluo-4 and Calcium 4 dyes, have relatively weak fluorescence signals limiting their application in high-throughput screening applications. Quest Fluo-8™ dye offers superior measurement of challenging cell lines and receptors.

Features	Benefits
Load dye in live cells at room temperature (or 37°C)	Room temperature is more compatible with HTS applications
Homogeneous mix-and-read, no-wash calcium mobilization assay	Washing cells introduces errors
Significantly brighter fluorescence intensity	2X brighter than Fluo-4, Fluo-4 Direct, Fluo-4 NW, FLIPR® Calcium 4 assay kits 4X brighter than Fluo-3
EC₅₀ values (half maximal effective concentration)	Comparable with Fluo-3, Fluo-4, Fluo-4 Direct™ and FLIPR® Calcium assay kits
No quencher dye	No potential interference from compound ligand-receptor interactions
Large assay window compared to other dyes	Easily detects low calcium mobilization in challenging cell lines and receptors
Maximum excitation at ~490 nm	Readily adopted into established protocols and instrument workflows
Rigorous Quality Control	Controls and eliminates non-specific assay artifacts

Summary

Compared to other commercial calcium assay kits based on either Fluo-3 or Fluo-4, our Screen Quest™ Calcium Assay Kits have the following advantages for HTS applications:

Broad Applications: work with both GPCR and calcium channel targets.
Convenient Spectral Wavelengths: maximum excitation @ ~490 nm; maximum emission @ ~514 nm.
Faster Dye Loading: dye loading at room temperature (rather than 37 ºC required for Fluo-4 AM).
No Wash Required and No Quencher Interference with Your Targets.
Robust Performance: enable calcium assays that are impossible with Fluo-4 AM or Fluo-3 AM.
Strongest Signal Intensity: 2 times brighter than Fluo-4 AM; 4 times brighter than Fluo-3 AM.