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Quest™ Fluo-8 Calcium Assay Kit for Cellular Analysis


Info

Ideal as calcium assay, brighter than Fluo-3 and Fluo-4.
Utilizing the brightest and most sensitive fluorescent Ca2+ indicator, Screen Quest(TM) Fluo-8 Calcium Assay Kit provides much higher signal intensity and very large fluorescence intensity increase in response to Ca2+ binding. AAT Bioquest’s homogeneous fluorescence-based assay kit detects calcium mobilization across a broad spectrum of biological targets. Easy-to-use protocol does not require a wash step or exogenous addition of a quencher dye, which could adversely affect receptor-ligand interaction kinetics.

Other widely used calcium ion indicators, such as Fluo-3, Fluo-4 and Calcium 4 dyes, have relatively weak fluorescence signals limiting their application in high-throughput screening applications. Quest Fluo-8™ dye offers superior measurement of challenging cell lines and receptors.

Features

Benefits

Load dye in live cells at room temperature (or 37°C)

Room temperature is more compatible with HTS applications

Homogeneous mix-and-read, no-wash calcium mobilization assay

Washing cells introduces errors

Significantly brighter fluorescence intensity

2X brighter than Fluo-4, Fluo-4 Direct, Fluo-4 NW, FLIPR® Calcium 4 assay kits
4X brighter than Fluo-3

EC50 values (half maximal effective concentration)

Comparable with Fluo-3, Fluo-4, Fluo-4 Direct™ and FLIPR® Calcium assay kits

No quencher dye

No potential interference from compound ligand-receptor interactions

Large assay window compared to other dyes

Easily detects low calcium mobilization in challenging cell lines and receptors

Maximum excitation at ~490 nm

Readily adopted into established protocols and instrument workflows

Rigorous Quality Control

Controls and eliminates non-specific assay artifacts



Summary


Compared to other commercial calcium assay kits based on either Fluo-3 or Fluo-4, our Screen Quest™ Calcium Assay Kits have the following advantages for HTS applications:
  • Broad Applications: work with both GPCR and calcium channel targets.
  • Convenient Spectral Wavelengths: maximum excitation @ ~490 nm; maximum emission @ ~514 nm.
  • Faster Dye Loading: dye loading at room temperature (rather than 37 ºC required for Fluo-4 AM).
  • No Wash Required and No Quencher Interference with Your Targets.
  • Robust Performance: enable calcium assays that are impossible with Fluo-4 AM or Fluo-3 AM.
  • Strongest Signal Intensity: 2 times brighter than Fluo-4 AM; 4 times brighter than Fluo-3 AM.

Order Information


Click here for Product Listing (see these very affordable prices!)

Read more:


Quest Fluo-8™ Calcium Reagents and Screen Quest™ Fluo-8 NW Calcium Assay Kits (pdf)


Protocols:


Related Calcium Detection Product for Red Fluorescence:
Rhod-4: The Brightest and Most Sensitive Red Fluorescent Ca2+ Indicator

More calcium detection products ...

 



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