Cell Meter(TM) Generic Fluorometric Caspase Activity Assay Kit *Optimized Red Fluorescence for Flow

Cell Meter(TM) Generic Fluorometric Caspase Activity Assay Kit *Optimized Red Fluorescence for Flow
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-22822 1 kit (100 assays) -

3 - 8 business days*

279.00€
 
The Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a... more
Product information "Cell Meter(TM) Generic Fluorometric Caspase Activity Assay Kit *Optimized Red Fluorescence for Flow"
The Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring generic caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) activation in living cells. Caspases activation is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). This kit uses TF5-VAD-FMK as a fluorogenic indicator for most caspase activity. TF5-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated casepase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the red fluorescent reagent is retained within the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. The reagent will start to react with active caspase enzymes within 15 minutes of addition to the media. The kit provides all the essential components with an optimized assay protocol. It is used for the quantification of most activated caspases activities in apoptotic cells, or for screening caspases inhibitors.
Supplier: AAT Bioquest
Supplier-Nr: 22822

Properties

Application: Caspase activation detection, FC
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Signal Word: Warning
Caution
Our products are for laboratory research use only: Not for administration to humans!
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