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 In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments are aiming to achieve high-resolution, multimodality and lifetime-based in vivo fluorescence imaging. Our iFluor™ 780 is designed to label proteins and other biomolecules with near infrared fluorescence. Conjugates prepared with iFluor™ 780 have the excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800 dye, with 783/814 nm excitation/emission maxima. iFluor™ 780 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7 or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or for other imaging applications that require NIR detections such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system. (Read more in this technical information sheet (pdf).) Amines react with carbonyl groups. Maleimides react with thiol groups. Hydrazides react with carbonyl groups. Succinimidyl ester react with amino groups.
See also: Superior Tide Fluor Dyes Classic Fluorescent Labeling Dyes and Quenchers |
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