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Hersteller INFOS

Classic Fluorescent Labeling Dyes and Quenchers

Fluorescence is the result of a three-stage process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes.


A fluorescent probe is a fluorophore designed to localize within a specific region of a biological specimen or to respond to a specific stimulus. Fluorescent probes enable researchers to detect particular components of complex biomolecular assemblies (including live cells) with exquisite sensitivity and selectivity. Reactive fluorescent dyes are widely used to modify amino acids, peptides, proteins (in particular, antibodies), oligonucleotides, nucleic acids, carbohydrates and other biological molecules. AAT Bioquest provides a full spectrum of fluorophores for labeling biopolymers and derivatizing low molecular weight molecules. Among the reactive dyes, amine-reactive dyes are most often used to prepare various bioconjugates for immunochemistry, histochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor binding and other biological applications since amino groups are either abundant or easily introduced into biomolecules. In general, thiol-reactive reagents are frequently used to develop probes for investigating some particular protein structures and functions. Additionally, some amine-containing fluorescent reagents are also used to modify biomolecules, in particular, to label glycoproteins. In general, the preferred bioconjugates should have high fluorescence quantum yields and retain the biological activities of the unlabeled biomolecules. It is quite critical to properly control the degree of substitution (DOS) when conducting a conjugation of biopolymers. A high degree of labeling may significantly decrease the water solubility and binding affinity/specificity of the target biomolecules. Although conjugating dyes to biomolecules is usually easy, preparing the optimal conjugate may require extensive experimentation. Fortunately there are some excellent publications that may provide you some important guidelines (For the technical details please read the references listed on the end of this section).
 

Amine-Reactive Fluorescent Dyes

Amine-reactive fluorescent probes are widely used to modify peptides, proteins, oligonucleotides, nucleic acids, ligands and other biomolecules. Amine-reactive dyes are most often used to prepare bioconjugates for immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling and fluorescent analog cytochemistry. In these applications, the stability of the chemical bond between the amine-reactive dye and biomolecule is particularly important because the fluorescent conjugates are often subjected to rigorous incubation, hybridization and washing steps. A number of fluorescent amino-reactive dyes have been developed to label various biomolecules, and the resultant conjugates are widely used in biological applications. Three major classes of amine-reactive fluorescent reagents are currently used to label biopolymers: succinimidyl esters (SE), isothiocyanates, and sulfonyl chlorides. AAT Bioquest offers all the popular amine-reactive fluorescent dyes for peptide/protein labelings, nucleotide modifications and microarray applications. Although FITC (fluorescein isothiocyanate), one of the most popular fluorescent labeling dyes, is predominantly used for preparing a variety of fluorescent bioconjugates, the low conjugation efficiency of FITC and the short life time of its conjugates are still troublesome for some critical biological applications. We strongly recommend that you choose succinimidyl esters for labeling needs if other conditions and factors are equivalent.

 

Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters

 

Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters
Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines. There are a few factors that should be considered when SE compounds are used for conjugation reaction:

 

 

Fluorescent Dye Sulfonyl Chlorides

 

Fluorescent Dye Sulfonyl Chlorides
Sulfonyl chlorides are highly reactive. These reagents are unstable in water, especially at the higher pH required for reaction with aliphatic amines. Molecular modifications by sulfonyl chlorides need to be carefully carried out preferably at low temperature. Sulfonyl chlorides can also react with phenols (including tyrosine), aliphatic alcohols (including polysaccharides), thiols (such as cysteine) and imidazoles (such as histidine), but these reactions are not common in proteins or in aqueous solution. There are a few factors that need to be considered when SC compounds are used for conjugation reaction:

 

 

Fluorescent Dye Isothiocyanates

 

Fluorescent Dye Isothiocyanates
Isothiocyanates form thioureas upon reaction with amines. It is proven that some thiourea products (in particular, the conjugates from α-amino acids/peptides/proteins) are much less stable than the conjugates that are prepared from the corresponding succinimidyl esters. It has been reported that antibody conjugates prepared from fluorescein isothiocyanates deteriorate over time. We strongly recommend that you use succinimidyl esters for your conjugations whenever possible. There are a few factors that need to be considered when SE compounds are used for conjugation reaction:

 

 

Other Amine-Reactive Fluorescent Reagents

Electron-deficient aryl halides are effective amine-reactive labeling reagents for the preparation of various bioconjugates. AAT Bioquest offers fluorescamine, NBD chloride, NBD fluoride, AAT fluoride, SBD fluoride, 5- and 6-fluorescein dichlorotriazines. Due to their low selectivity, these dyes also react with thiol moieties besides amino groups. 5- and 6-fluorescein dichlorotriazines may even react with hydroxy compounds such as carbohydrates.
Non-fluorescent NBD chloride readily reacts with primary aliphatic amines (such as amino acids), generating bright yellow fluorescent amine adducts. NBD also reacts with thiols, although these adducts absorb and emit at shorter wavelengths and are less fluorescent than amine derivatives. NBD fluoride usually yields the same products as NBD chloride but is much more reactive. NBD fluoride may even react with hydroxy group under harsh conditions when amino or thiol groups are not available. The absorption and fluorescence emission spectra, quantum yields and extinction coefficients of NBD conjugates are all markedly dependent on the surrounding environment. Like the Dansyl dye adducts, the fluorescence quantum yield of NBD adducts of amines in water are very low (<0.01). NBD adducts of secondary amines are less fluorescent than that of primary amines while the adducts of aromatic amines with NBD are essentially non-fluorescent. There are a few factors that need to be considered when electron-deficient aryl halides are used for conjugation reaction.

 

 

Thiol-Reactive Fluorescent Dyes

Because free thiol (SH) groups, also called mercapto groups, are not present as abundantly as amino groups in most biopolymers such as proteins and nucleic acids, thiol-reactive reagents often provide a means of selectively modifying a protein at a defined site. Therefore thiol-reactive dyes are often used to prepare fluorescent peptides, proteins and oligonucleotides for probing biological structures, functions and interactions. Thiol-reactive dyes have been used to develop probes for analyzing the topography of proteins in biological membranes, determining distances within the protein or between the proteins and monitoring the changes in protein conformation using environment-sensitive probes.

There are many types of thiol-reactive dyes reported in the literature, including iodoacetamides, disulfides, maleimides, vinyl sulfones and various electron-deficient aryl halides and sulfonates. Iodoacetamides and maleimides are by far the most popular thiol-reactive moieties.

 

Fluorescent Dye Iodoacetamides (IA)

 

Fluorescent Dye Iodoacetamides (IA)
Iodoacetamides are one of the most popular thiol-reactive moieties for labeling biopolymers and small biomolecules. Iodoacetamides readily react with thiol moieties of biopolymers and small biomolecules to form thioether conjugates. The thioether bond formed is quite stable. Although iodoacetamides generally have good selectivity to thiol groups, they may react with histidine or potentially tyrosine under higher pH if free thiols are not readily available. The bioconjugation reactions of thiol-reactive probes can be quenched by the addition of cysteine, glutathione or mercaptosuccinic acid to the reaction mixture, forming highly water-soluble adducts that are easily removed by dialysis or gel filtration. There are quite a few factors that need to be considered when iodoacetamides are used for conjugation reaction:

 

 

Fluorescent Dye Maleimides

 

Fluorescent Dye Maleimides
Maleimides and iodoacetamides are by far the most popular thiol-reactive moieties. Maleimides readily react with thiol moieties of biopolymers to form thioether conjugates even under neutral conditions. The thioether bond formed is quite stable. Maileimides are generally much less light-sensitive than iodoacetamides. The latter compounds are known to be very light liable, especially in solution.

Maleimides require conjugation conditions less stringent than those of iodoacetamides as described above. Unlike iodoacetamides, maleimides do not react with histidine and methionine under physiological conditions. For example, most conjugations can be done at room temperature at neutral pH. However, either elevated or reduced pH or temperature may be required for a particular labeling reaction.
 

Other Thiol-Reactive Fluorescent Dyes

NBD chloride was first introduced as a fluorogenic derivatization reagent for amines. It also reacts with thiols to form adducts that absorb and emit at shorter wavelengths. NBD and SBD compounds are widely used for fluorogenic thiol modifications of biopolymers. In general, NBD compounds are more often used for modifying amino groups and SBD compounds for selective modifications of thiol groups. The absorption and fluorescence emission spectra, quantum yields and extinction coefficients of NBD-thiol conjugates are dependent on the surrounding environment. SBD is an analog of NBD. SBD fluoride has been used for the derivatization of both amino and thiol groups. Quite a few excellent reviews have been published for the applications of SBD, NBD and SBD compounds (see the reference listed on the end of this chapter). Most of NBD and SBD compounds react with both amino and thiol groups except that SBD-Cl has good selectivity to thiol groups. Additionally, NBD and SBD compounds are widely used for HPLC derivatizations.
Bromobimanes including monobromobimane and dibromobimane are another class of popular thiol-reactive fluorescent tags, and is widely used to detect various thiol-containing biomolecules such as glutathione in cells. It is fluorogenic upon reacting with thiol-containing molecules. The monobromobimanes are essentially non-fluorescent until they react with several low molecular weight thiols, including glutathione, N-acetylcysteine, mercaptopurine, peptides and plasma thiols, as well as with carboxylic acids. Monobromobimane is the most extensively used bimane derivative. These reagents are also useful for detecting the distribution of protein thiols in cells before and after chemical reduction of disulfides. Both monobromobimane and the more thiol-selective monochlorobimane have been extensively used for detecting glutathione in live cells. Monobromobimane can also be used to derivatize thiol-containing proteins prior to separation by isoelectric focusing without appreciably modifying the proteins electrophoretic mobility. Dibromobimane is an interesting crosslinking reagent for proteins because it is unlikely to fluoresce until both of its alkylating groups have reacted.
 

Carbonyl-Reactive (Amine-Containing) Fluorescent Dyes and Their Applications

Amine-containing dyes are widely used to modify water-soluble biopolymers (such as proteins) through the formation of Schiff Base or reductive amination. Among them, fluorescently labeled cadaverine and lysine derivatives and a variety of hydrazides have been predominantly used for modifying biomolecules. These dyes are used for modifications of carbohydrates, glycoproteins and nucleic acids that are first periodate-oxidized to introduce aldehydes and ketones into the biopolymers for subsequent reductive amination. The combination of periodate oxidation with reductive amination provides an effective way for site-selective modifications of biopolymers. For example, periodate oxidation of the 3-terminal ribose is reported to be one of the few methods of selectively modifying RNA. Periodate-oxidized ribonucleotides are converted to fluorescent nucleotide probes by reaction with fluorescent hydrazines and amines.
Amine-containing dyes are also used to modify biopolymers (such as proteins) using water-soluble carbodiimides (such as EDC) to convert the carboxy groups of the biopolymers into amide groups. Either NHS or NHSS may be used to improve the coupling efficiency of EDC-mediated protein–carboxylic acid conjugations. A large excess of the amine-containing dyes is usually used for EDC-mediated bioconjugations in concentrated protein solutions at low pH to reduce intra- and inter-protein coupling to lysine residues, a common side reaction.
The amine-containing dyes are also valuable building blocks in bioorganic and medicinal chemistry. We have used our amine-containing dyes to custom-synthesize many fluorescently labeled drugs, natural toxins and biological ligands.
 

Classic Dyes

 

Coumarins

Cat #
Size
Product Name
ABD-501
25 mg
AMCA acid
ABD-502
10 mg
AMCA, succinimidyl ester
ABD-505
100 mg
DEAC [7-Diethylaminocoumarin-3-carboxylic acid]
ABD-506
25 mg
DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester]
ABD-554
100 mg
7-Hydroxy-4-methylcoumarin-3-acetic acid
ABD-556
25 mg
7-Hydroxy-4-methylcoumarin-3-acetic acid, succinimidyl ester
ABD-550
250 mg
7-Hydroxycoumarin-3-carboxylic acid
ABD-551
50 mg
7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester
ABD-557
1 g
MCA [7-Methoxycoumarin-4-acetic acid]
ABD-558
25 mg
MCA succinimidyl ester [7-Methoxycoumarin-4-acetic acid, succinimidyl ester]
ABD-560
1 g
7-Methoxycoumarin-3-carboxylic acid
ABD-561
5 g
7-Methoxycoumarin-3-carboxylic acid
ABD-563
100 mg
7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester

 

Fluoresceins

Cat #
Size
Product Name
ABD-206
25 mg
 AMF [4’-(Aminomethyl)fluorescein]
ABD-200
25 mg
 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein]
ABD-201
10 g
 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein]
ABD-205
1 g
 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein]
ABD-106
100 mg
 6-FAM [6-Carboxyfluorescein] *Single isomer*
ABD-107
1 g
 6-FAM [6-Carboxyfluorescein] *Validated for labeling oligos*
ABD-108
5 g
 6-FAM [6-Carboxyfluorescein] *Validated for labeling oligos*
ABD-127
100 mg
 5(6)-FAM cadaverine
ABD-128
100 mg
 5-FAM cadaverine
ABD-123
100 mg
 5(6)-FAM ethylenediamine
ABD-124
100 mg
 5-FAM ethylenediamine
ABD-110
25 mg
 5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *Mixed isomers*
ABD-111
100 mg
 5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *Validated for labeling peptides and oligos*
ABD-112
1 g
 5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *Validated for labeling peptides and oligos*
ABD-113
10 mg
 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *Single isomer*
ABD-114
100 mg
 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *Validated for labeling peptides*
ABD-115
1 g
 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *Validated for labeling peptides*
ABD-116
10 mg
 6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *Single isomer*
ABD-117
100 mg
 6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *Validated for labeling oligos*
ABD-118
1 g
 6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *Validated for labeling oligos*
ABD-120
100 mg
 5-FITC [FITC Isomer I; fluorescein-5-isothiocyanate] *UltraPure Grade*
ABD-121
1 g
 5-FITC [FITC Isomer I; fluorescein-5-isothiocyanate] *UltraPure Grade*
ABD-122
10 g
 5-FITC [FITC Isomer I; fluorescein-5-isothiocyanate] *UltraPure Grade*
ABD-129
100 mg
 5-FITC cadaverine
ABD-130
25 mg
 Fluorescein-5-maleimide
ABD-222
25 mg
 5-IAF [5-Iodoacetamidofluorescein]
ABD-203
5 mg
 6-JOE, SE [6-Carboxy-4,5-dichloro-2,7-dimethoxyfluorescein, succinimidyl ester]

 

Rhodamines

Cat #
Size
Product Name
ABD-320
100 mg
5(6)-CR110 [5-(and 6)-Carboxyrhodamine 110] *Mixed isomers*
ABD-321
1 g
5(6)-CR110 [5-(and 6)-Carboxyrhodamine 110] *Mixed isomers*
ABD-322
5 mg
5-CR110 [5-Carboxyrhodamine 110] *Single isomer*
ABD-323
5 mg
6-CR110 [6-Carboxyrhodamine 110] *Single isomer*
ABD-330
25 mg
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]
ABD-331
10 mg
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*
ABD-332
10 mg
6-CR6G [6-Carboxyrhodamine 6G] *Single isomer*
ABD-340
10 mg
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*
ABD-341
1 mg
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
ABD-345
5 mg
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
ABD-342
1 mg
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
ABD-346
5 mg
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
ABD-479
1 mg
California Red™ SE
ABD-343
1 mg
5(6)-Caroxyrhodamine 6G cadaverine
ABD-344
1 mg
5(6)-Caroxyrhodamine 6G ethylenediamine
ABD-470
100 mg
Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride]
ABD-380
100 mg
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *Mixed isomers*
ABD-381
5 g
5-ROX [5-Carboxy-X-rhodamine] *Single isomer*
ABD-382
25 mg
6-ROX [6-Carboxy-X-rhodamine] *Single isomer*
ABD-390
25 mg
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers*
ABD-391
1 g
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *Single isomer*
ABD-392
5 mg
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *Single isomer*
ABD-480
10 mg
Sulforhodamine 101 sulfonyl chloride
ABD-394
5 mg
Sunnyvale Red™ SE *Superior 6-ROX Replacement*
ABD-360
100 mg
5-(and 6)-TAMRA [5-(and-6)-Carboxytetramethylrhodamine] *Mixed isomers*
ABD-361
1 g
5-(and 6)-TAMRA [5-(and-6)-Carboxytetramethylrhodamine] *Validated for labeling peptides and oligos*
ABD-362
5 g
5-(and 6)-TAMRA [5-(and-6)-Carboxytetramethylrhodamine] *Validated for labeling peptides and oligos*
ABD-363
10 mg
5-TAMRA [5-Carboxytetramethylrhodamine] *Single isomer*
ABD-364
100 mg
5-TAMRA [5-Carboxytetramethylrhodamine] *Validated for labeling peptides*
ABD-365
1 g
5-TAMRA [5-Carboxytetramethylrhodamine] *Validated for labeling peptides*
ABD-366
10 mg
6-TAMRA [6-Carboxytetramethylrhodamine] *Single isomer*
ABD-367
100 mg
6-TAMRA [6-Carboxytetramethylrhodamine] *Validated for labeling oligos*
ABD-368
1 g
6-TAMRA [6-Carboxytetramethylrhodamine] *Validated for labeling oligos*
ABD-355
25 mg
5(6)-TAMRA cadaverine
ABD-356
5 mg
5-TAMRA cadaverine
ABD-357
5 mg
6-TAMRA cadaverine
ABD-354
25 mg
5(6)-TAMRA ethylenediamine
ABD-358
5 mg
5-TAMRA ethylenediamine
ABD-359
5 mg
6-TAMRA ethylenediamine
ABD-370
25 mg
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *Mixed isomers*
ABD-371
100 mg
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *Validated for labeling peptides and oligos*
ABD-372
1 g
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *Validated for labeling peptides and oligos*
ABD-373
5 mg
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *Single isomer*
ABD-374
100 mg
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *Validated for labeling peptides*
ABD-375
1 g
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *Validated for labeling peptides*
ABD-377
100 mg
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *Validated for labeling oligos*
ABD-376
5 mg
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *Single isomer*
ABD-378
1 g
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *Validated for labeling oligos*
ABD-423
5 mg
5(6)-TMR C6 malemide
ABD-424
5 mg
5-TMR C6 malemide
ABD-425
5 mg
6-TMR C6 malemide
ABD-483
5 mg
TR C2 malemide
ABD-482
5 mg
TR cadaverine [Sulforhodamine 101 cadaverine sulfonamide]
ABD-410
5 mg
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate]
ABD-415
1 mg
5-TRITC; G isomer [Tetramethylrhodamine-5-isothiocyanate]
ABD-417
1 mg
6-TRITC; R isomer [Tetramethylrhodamine-6-isothiocyanate]
ABD-412
5 mg
Tetramethylrhodamine-5-(and-6)-maleimide *Mixed isomers*
ABD-421
1 mg
Tetramethylrhodamine-5-maleimide *Single isomer*
ABD-419
1 mg
Tetramethylrhodamine-6-maleimide *Single isomer*

 

Other Dyes

Cat #
Size
Product Name
ABD-634
5 mg
bBBr [Dibromobimane] *UltraPure Grade*
ABD-633
25 mg
mBBr [Monobromobimane] *UltraPure Grade*
ABD-635
10 mg
mBCl [Monochlorobimane] *UltraPure Grade*
ABD-810
25 mg
Dansyl cadaverine [5-Dimethylaminonaphthalene-1-(N-(5-aminopentyl))sulfonamide]
ABD-811
100 mg
Dansyl chloride [5-Dimethylaminonaphthalene-1-sulfonyl chloride]
ABD-813
1 g
Dansyl-X, SE
ABD-812
5 g
Dansyl-X, acid
ABD-617
25 mg
EDANS C2 maleimide
ABD-610
1 g
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]
ABD-611
10 g
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]
ABD-618
25 mg
EDANS iodoacetamide [5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid]
ABD-615
1 g
EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt]
ABD-616
10 g
EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt]
ABD-820
25 mg
Fluorescamine *UltraPure Grade*
ABD-825
25 mg
NBD-Cl [4-Chloro-7-nitrobenzofurazan] *UltraPure grade*
ABD-821
5 mg
NBD-F [4-Fluoro-7-nitrobenzofurazan] *UltraPure grade*
ABD-828
1 g
NBD-X acid
ABD-829
100 mg
NBD-X, succinimidyl ester
ABD-700
10 mg
Pyrromethene FL
ABD-701
5 mg
Pyrromethene FL, SE

 

Classic Quenchers

Cat #
Size
Product Name
ABD-2006
100 mg
DABCYL C2 amine
ABD-2008
25 mg
DABCYL C2 maleimide
ABD-2001
5 g
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *UltraPure grade*
ABD-2002
25 g
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *UltraPure grade*
ABD-2003
100 g
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *UltraPure grade*
ABD-2004
1 g
DABCYL succinimidyl ester [4-((4-(Dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester]
ABD-2005
5 g
DABCYL succinimidyl ester [4-((4-(Dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester]
ABD-2030
1 g
DABSYL chloride [4-Dimethylaminoazobenzene-4-sulfonyl chloride] *UltraPure grade*
ABD-2025
25 mg
DNP amine
ABD-2026
25 mg
DNP maleimide
ABD-2020
100 mg
DNP-X acid [6-(2,4-Dinitrophenyl)aminohexanoic acid]
ABD-2021
25 mg
DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester]



Overview: Classic Reactive Fluorescent Labeling Dyes & Their Applications (pdf)


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