IHC Substrates and Counterstains

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Counterstain and substrate compability table:
| | Blue Counterstain | Red Counterstain | | Substrate (Catalog No.), color | Mayers Hematoxylin Solution (S-99025), intensive blue Nuclear Blue Solution (S-99035), light blue | Nuclear Fast Red Solution (S-99031), intensive red Nuclear Red Solution (S-99033), light red | AP-Detection | AP-Red Solution Set ISH (S-99061), red | excellent contrast | color incompatibility | AP-Red Solution Set IHC (S-99065), red | excellent contrast | color incompatibility | NBT/BCIP Solution (S-99064), dark blue/violet | color incompatibility | excellent contrast | HRP-Detection | AEC Solution ISH* (S-99010), red | excellent contrast | color incompatibility | AEC Solution IHC* (S-99012), red | excellent contrast | color incompatibility | DAB Solution Set (S-99014), brown | excellent contrast | fair contrast | HRP-Green Solution Set (S-99056), green | good contrast | excellent contrast |
*The reaction product is soluble in alcohol. Thus, aqueous counterstains and mounting media must be used.
Also available: 42 Life Sciences ready-to-use pepsin solution in stabilizing buffer.
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42 Life Sciences: IHC Product Overview
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