HRP-Green Solution Set (2 components):-
The HRP-Green Solution Set is a new chromogen used in immunohistochemical and chromogenic in situ hybridization applications. The HRP-Green Solution Set is supplied as two liquid components, the buffer and the chromogen solution, which are mixed together before use. Stability of the working solution is at least 4 hours at room temperature. In the presence of horseradish peroxidase (HRP) HRP-Green Solution deposits an intense green precipitate. HRP-Green shows an excellent contrast with red alkaline phophatase subtrates. The reaction product is insoluble in alcohols and xylene and, therefore, a variety of counterstains and permanent mounting media can be used with HRP-Green.
The HRP-Green Solution Set provides a two component liquid substrate system that deposits an intense green stain in the presence of HRP.
Recommended Guidelines For Use
Bring reagents to room temperature before use
Do not exceed incubation times of 30 s per washing and dehydration step. Use only high purity xylene at the end of the dehydration series. In double staining procedures, HRP-Green should be used as the last chromogen. Occasionally HRP-Green Solutions may precipitate, which does not affect staining
HRP-Green Solution Set Order Information