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Nuclear Red Solution

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Ready-to-use Nuclear Red Solution.


 

Introduction

Nuclear Red Solution is a nuclear fast red (a.k.a. Kernechtrot) based reagent for counterstaining applications in in situ hybridization (ISH) and immunohistochemistry (IHC) procedures.

Nuclear Red Solution (ready-to-use) is optimized to avoid overstaining of nuclei and for in situ hybridization (ISH) and immunohistochemistry (IHC) procedures using common chromogens like BCIP/NBT. Nuclei will be stained light red-pink. Cytoplasm will be light yellow or unstained.
Specimens counterstained with Nuclear Red Solution should be dehydrated and mounted with a xylene-based mounting media. This counterstain is not compatible with aqueous mounting media.
 

Description

Nuclear Red Solution contains a nuclear fast red stain in stabilizing buffer. No dilution is required prior to use.
 

Recommended Guidelines For Use

  1. Rinse slides with reagent quality water.
  2. Counterstain tissue samples for approx. 2 min with Nuclear Red Solution.*
  3. Transfer slides into a staining jar and wash 1 min with reagent quality water.
  4. Dehydrate in an ascending ethanol dilution series (70-95-2x 100%).
  5. Clear 2 x 2 min in xylene, then air dry.
  6. Mount slides with xylene-based mounting solution.

* Time depends on the nature of tissue/cell and should be optimized.

Manual


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