Nuclear Red Solution is a nuclear fast red (a.k.a. Kernechtrot) based reagent for counterstaining applications in in situ hybridization (ISH) and immunohistochemistry (IHC) procedures.
Nuclear Red Solution (ready-to-use) is optimized to avoid overstaining of nuclei and for in situ hybridization (ISH) and immunohistochemistry (IHC) procedures using common chromogens like BCIP/NBT. Nuclei will be stained light red-pink. Cytoplasm will be light yellow or unstained.
Specimens counterstained with Nuclear Red Solution should be dehydrated and mounted with a xylene-based mounting media. This counterstain is not compatible with aqueous mounting media.
Nuclear Red Solution contains a nuclear fast red stain in stabilizing buffer. No dilution is required prior to use.
* Time depends on the nature of tissue/cell and should be optimized.