Nuclear Fast Red Solution is designed for use as a counterstaining solution in immunohistochemistry (IHC) procedures using common chromogens like BCIP/NBT.
Nuclei will be stained red-pink. Cytoplasm will be yellow or unstained.
Specimens counterstained with Nuclear Fast Red Solution should be dehydrated and mounted with a xylene-based mounting media. This counterstain is not compatible with aqueous mounting media.
Nuclear Fast Red Solution contains a nuclear fast red stain (a.k.a. Kernechtrot) in stabilizing buffer. No dilution is required prior to use.
* Time depends on the nature of tissue/cell and should be optimized.