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Nuclear Fast Red Solution

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Ready-to-use Nuclear Fast Red Solution.


 

Introduction

Nuclear Fast Red Solution is designed for use as a counterstaining solution in immunohistochemistry (IHC) procedures using common chromogens like BCIP/NBT.

Nuclei will be stained red-pink. Cytoplasm will be yellow or unstained.
Specimens counterstained with Nuclear Fast Red Solution should be dehydrated and mounted with a xylene-based mounting media. This counterstain is not compatible with aqueous mounting media.
 

Description

Nuclear Fast Red Solution contains a nuclear fast red stain (a.k.a. Kernechtrot) in stabilizing buffer. No dilution is required prior to use.
 

Recommended Guidelines For Use

  1. Rinse slides with reagent quality water.
  2. Counterstain tissue samples for 1-10 min with Nuclear Fast Red Solution.*
  3. Transfer slides into a staining jar and wash 1 min with reagent quality water.
  4. Dehydrate in an ascending ethanol dilution series (70-95-2x 100%).
  5. Clear 2 x 2 min in xylene, then air dry.
  6. Mount slides with xylene-based mounting solution.

* Time depends on the nature of tissue/cell and should be optimized.

Manual


Ready-to-use Nuclear Fast Red Solution Order Info

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