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Mayer's Hematoxylin Solution


Info

Ready-to-use Mayers Hematoxylin Solution in stabilizing buffer.
  • one stable component
  • optimized for immunohistochemical (IHC) procedures
  • non-toxic

Introduction


Mayers Hematoxylin Solution is a staining reagent for histological applications. Nuclei in stained sections will be a blue-violet color with crisp nuclear detail. This reagent is suitable for use with all chromogens commonly used in immunohistochemical (IHC) applications.

Description


Ready-to-use Mayer‘s Hematoxylin Solution in stabilizing buffer. Does not contain chloralhydrate. Optimized for the use in immunohistochemical (IHC) procedures.

Recommended Guidelines For Use

  1. Rinse slides with tap water
  2. Counterstain tissue or cell samples for 10-60 s with Mayer’s Hematoxylin Solution.*
  3. Transfer slides into a staining jar and wash 2 min with running tap water.
  4. Dehydrate in an ascending ethanol dilution series (70-85-95-100%).
  5. Incubate 2 x 2 min in xylene, then air dry.
  6. Mount slides with Mounting Solution.
Alternatively, mount in aqueous-based mounting solution after blueing.
* Time depends on the nature of tissue/cell and should be optimized.

Manual
Ready-to-use Mayer's Hematoxylin Solution Order Information

 



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