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AEC Solution for ISH

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Powerful ready-to-use chromogenic substrate solution for HRP in in situ hybridization (ISH) procedures.


 

Introduction

AEC (3-Amino-9-Ethylcarbazole) is a commonly used chromogene for immunohistochemical (IHC) and in situ hybridization (ISH) procedures. Upon reaction with peroxidase AEC produces a red colored deposit that is soluble in alcohol. Thus, aqueous counterstain and mounting media must be used.
 

Description

Ready-to-use AEC solution in stabilizing buffer. Contains hydrogen peroxide. Optimized for the use in in-situ hybridization (ISH) procedures to result in intensive staining with low background.
 

Recommended Guidelines For Use

  1. Check AEC solution. In case of precipitates (not affecting quality) filtrate solution using a standard filter.
  2. Cover specimen, which has been reacted with peroxidase conjugate, with AEC Solution.
  3. Incubate for approx. 5-30 minutes * at room temperature.
  4. To stop color development wash the slide in tap water.
  5. If desired counterstain with e.g. Mayers hematoxylin then rinse in tap water.
  6. Mount slide with aqueous mounting medium.

* Monitor color development with a microscope.

Manual


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