Optimized for in situ hybridization (ISH) and immunohistochemical (IHC) procedures refrigerator stable.
DAB (3,3‘-diaminobenzidine) is a widely used chromogen in immunohistochemical and immunoblotting applications. The DAB Solution Set is supplied as two liquid components, the buffer and the refrigerator stable chromogen solution, which are mixed together before use. Stability of the working solution is at least 6 hours at room temperature. In the presence of horseradish peroxidase (HRP) DAB deposits an intense brown precipitate. The reacti-on product is insoluble in al-cohols and xylene and, there-fore, a variety of coun-terstains and permanent mounting media can be used with DAB.
The DAB Solution Set provides a two component liquid substrate system that deposits an intense brown specific stain in the presence of HRP.
Bring reagents to room temperature before use
Occasionally DAB Chromogen may be hazy. The haziness may be removed by filtering the solution.