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Pepsin Solution


This ready-to-use pepsin solution in stabilizing buffer is a single component solution, which is stable in the refrigerator (2 °C - 8 °C).

Pepsin is a commonly used digestive enzyme for immuno-histochemical (IHC) and in situ hybridization (ISH) procedures.

Most of the commonly used fixatives as e. g. formamide cause cross-linking of proteins, which leads to masking or denaturation of tissue antigens. Treatment of fixed tissue sections with proteolytic enzymes as e.g. pepsin is often required to unmask antigens for optimal staining in IHC procedures.

For ISH applications treatment with proteolytic enzyms is required to digest excess aldehyde linkages to enable DNA or RNA probes to penetrate the tissue sections.


Recommended Guidelines For Use

  1. Deparaffinize the tissue sections and hydrate to water.
  2. Remove excess water from the glass slide without letting the tissue dry.
  3. Cover the tissue section with Pepsin Solution.
  4. Incubate for approx. 10 minutes * at 37 °C.
  5. Wash in several changes of water or buffer.
  6. Proceed with immunostaining or in situ-hybdridization procedure.

* Depending on the type of tissue, fixation time, fixative etc.


Ready-to-use pepsin solution in stabilizing buffer

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