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HRP-Green Substrate


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The HRP-Green Solution Set is a new chromogen used in immunohistochemical and chromogenic in situ hybridization applications.

HRP-Green


HRP-Green is a unique 2-components chromogen specifically developed for use in chromogenic in situ hybridization (CISH) and immunohistochemical (IHC) staining methods where peroxidase (HRP) is the enzyme label.

HRP-Green is supplied as two liquid components, the buffer and the chromogen solution. Unlike conventional HRP-substrates developing green precipitates, the working solution is stable for at least 4 hours at room temperature! In contrast to the commonly used DAB chromogen, none of the HRP-Green components is suspected to be carcinogenic.

In the presence of horseradish peroxidase (HRP) HRP-Green deposits a green reaction product at the site of the target, which can be visualized with standard optical light microscopy. HRP-Green shows a nice contrast with alkaline phophatase subtrates and can be used with common counterstains as e.g. Mayers Hematoxylin or Nuclear Fast Red.

HRP-Green stained slides can be mounted permanently using xylene-based standard permanent mounting media.

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Recommended Guidelines For Use


Bring reagents to room temperature before use
  1. After incubation of tissue sections with HRP-labeled antibodies, rinse in an appropriate buffer.
  2. Mix 1 drop (30 µl) of HRP-Green Chromogen per 1 ml of HRP-Green Buffer. To intensify staining, up to 3 drops HRP-Green Chromogen per 1ml of HRP-Green Buffer can be used.
  3. Apply HRP-Green mixture to tissue sections.
  4. Incubate from 2-10 minutes at room temperature.
  5. Rinse tissue, counterstain, dehydrate and coverslip.
Do not exceed incubation times of 30 s per washing and dehydration step. Use only high purity xylene at the end of the dehydration series. In double staining procedures, HRP-Green should be used as the last chromogen. Occasionally HRP-Green Solutions may precipitate, which does not affect staining quality.

Manual

HRP-Green Order Info

 



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