Kontakt 0800-2466651 DE | EN Merkliste Login Warenkorb

HOPE® - Formalin-free Tissue Fixation


HOPE® is the formalin-free fixation technique for molecular pathology and research.

Tissue conservation using the new HOPE®-technique results in paraffin embedded tissue blocks with native structure of the macro-molecules (protein, DNA, RNA) in the tissue conserved.

During HOPE® fixation, tissue samples are incubated in HOPE® I solution, then in HOPE® II solution, followed by a dehydration with acetone and subsequent embedding in paraffin.

For this tissue processing no alcohols or aldehydes (formalin, glutaraldehyde) are used. As a consequence, there is no cross-linking of macro-molecular structures during the process. This opens up new opportunities to characterize tissues out of paraffin blocks.

Many "cryo-type" antibodies are working on HOPE® fixed tissue sections and many antibodies are working without any heat pretreatment or proteolytic digestion steps often necessary for formalin fixed tissue sections.

There is no "over-fixation" with HOPE®, as is known with formalin, when keeping the tissue specimen in the fixation solution. This results in a more standardized fixation process.

RNA, DNA and proteins are conserved in high quality, thus making subsequent applications possible like e.g. PCR, RT-PCR, Northern-Blot, Immunohistochemistry (IHC), in situ hybridization (ISH), Western-Blot (WB) and many others, out of the same tissue blocks.


HOPE® Protocols

Here you will find protocols for fixing tisssue with the HOPE®-technique and for subsequent applications when using HOPE®-fixed paraffin embedded tissues.

Complete Instructions for use (German/English) for tissue fixation and embedding using the HOPE®-technique.



What does "HOPE" mean?
HOPE® is the abbreviation for "HEPES-Glutamic Acid Buffer Mediated Organic Solvent Protection Effect". Tissue is protected by a HEPES/glutamic-acid buffer against negative effects of organic fixation solutions (e.g. acetone).

How should HOPE®-tissue blocks be stored?
We recommend storage of HOPE®-tissue blocks at 4°C. This ensures high quality conservation of protein, DNA and RNA for molecular pathology and research applications for many years.

HOPE® Product Listing ...

Native Glycosylation of Recombinant Human ProteinsSelective Protease Activity Imaging in 3DCONDA Chromogenic Media Special Offer

StressMarq Antibody Trial ProgramThe Importance of Internal StandardsTrueBlot for Optimum WB after IP alle Treffer anzeigen (389 INFOS)

zurück Hoch

Forschungsprodukte im Fokus
€ 10 Gutschein sichern