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Human Cell Growth Stimulation G-CSF


Authentic - human cell expressed - G-CSF (HuXp): More effective for human cell growth stimulation.
Granulocyte colony-stimulating factor (G-CSF), is a hematopoietic growth factor that stimulates the development of committeed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. Currently, commercially available G-CSF proteins are produced in an E coli expression system and typically have a molecular mass of 19kDa. HumanZyme Inc. scientists have produced G-CSF (HuXp) expressed in engineered human 293 cells. The proteins are glycosylated forms with a molecular mass range of 22-25 kDa. G-CSF (HuXp) can be cost-effectively produced at large scale.

The bioactivity of G-CSF (HuXp) was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells. G-CSF expressed in E. coli was compared to the human cell expressed version G-CSF (HuXp). The results indicate that G-CSF (HuXp) is 4-5 fold more active than the E. coli expressed cytokine and is more effective for stimulating cell growth.

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