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Human GM-CSF Comparison


GM-CSF (HuXp) recombinant human cytokine authentic - human cell expressed .
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic eosinophilic progenitor cells. This protein is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli.

Currently, commercially available GM-CSF proteins are produced from E coli. HumanZyme has produced GM-CSF (HuXp) from engineered human 293 cells. The E. coli expressed protein has a molecular mass of 14 kD. This compares with the GM-CSF (HuXp) which migrates as a broad band of 15-30 kD due to glycosylation and sialylation. The biological activity of GM-CSF was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. Both versions of GM-CSF were assayed for cell proliferation at 37ºC for seven days. As shown in the figure, GM-CSF (HuXp) has 10-fold higher potency, demonstrating that it has higher activity and greater stability in cell culture conditions after seven days than E. coli expressed cytokine.

Our technical report, "New Protocol for Efficient Generation of Immature Human Dendritic Cells with Authentic GM-CSF (HuXp) and IL-4 (HuXp)" discusses a preferred, time saving protocol.

Authentic recombinant human cytokines - human cell expressed ...

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