Primer

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen. In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung, welche die in vitro-Vervielfältigung von DNA ermöglicht. Die Vervielfältigung wird durch DNA-Polymerasen erreicht, denen die Primer als Startpunkt bei der DNA-Synthese dienen. Primer erlauben die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qRT-PCR (quantitative Real-Time PCR), die quantitative Detektion von Nukleinsäuren.

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen . In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung,... mehr erfahren »
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Primer

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen. In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung, welche die in vitro-Vervielfältigung von DNA ermöglicht. Die Vervielfältigung wird durch DNA-Polymerasen erreicht, denen die Primer als Startpunkt bei der DNA-Synthese dienen. Primer erlauben die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qRT-PCR (quantitative Real-Time PCR), die quantitative Detektion von Nukleinsäuren.

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TNF, Human tumor necrosis factor (TNF superfamily, member 2), Real Time PCR Primer Set
TNF, Human tumor necrosis factor (TNF superfamily, member...

Artikelnummer: VHPS-9415

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: TNF, TNFA, TNFA protein, Tumor necrosis factor-alpha, Tumor necrosis factor (TNF superfamily, member 2), cDNA, FLJ95875,...
Anwendung: RNA quantification
43,00 €
Bewerten
Mouse ER Stress (UPR) Primer Library
Mouse ER Stress (UPR) Primer Library

Artikelnummer: MEPR-I

Schlagworte: MEPR-I
Spezies-Reaktivität: mouse
854,00 €
Bewerten
LRRC3, Human leucine rich repeat containing 3, Real Time PCR Primer Set
LRRC3, Human leucine rich repeat containing 3, Real Time...

Artikelnummer: VHPS-5375

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: LRRC3, C21orf102, Leucine-rich repeat-containing protein 3
Anwendung: RNA quantification
43,00 €
Bewerten
NBP, Human, Real Time PCR Primer Set
NBP, Human, Real Time PCR Primer Set

Artikelnummer: VHPS-6064

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: p63, KET, p51, p40, CUSP, TP63, p73L, Tumor protein 63, Tumor protein p73-like, Transformation-related protein 63,...
Anwendung: RNA quantification
43,00 €
Bewerten
TP73L, Human, Real Time PCR Primer Set
TP73L, Human, Real Time PCR Primer Set

Artikelnummer: VHPS-9507

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: p63, KET, p51, p40, CUSP, TP63, p73L, Tumor protein 63, Tumor protein p73-like, Transformation-related protein 63,...
Anwendung: RNA quantification
43,00 €
Bewerten
TRIM29, Human tripartite motif-containing 29, Real Time PCR Primer Set
TRIM29, Human tripartite motif-containing 29, Real Time...

Artikelnummer: VHPS-9570

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: ATDC, TRIM29, Tripartite motif-containing protein 29, Ataxia telangiectasia group D-associated protein
Anwendung: RNA quantification
43,00 €
Bewerten
Dcn, Mouse decorin, Real Time PCR Primer Set
Dcn, Mouse decorin, Real Time PCR Primer Set

Artikelnummer: VMPS-1563

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Dcn, PG40, PG-S2, Decorin, Bone proteoglycan II
Anwendung: RNA quantification
43,00 €
Bewerten
Lrrc3, Mouse leucine rich repeat containing 3, Real Time PCR Primer Set
Lrrc3, Mouse leucine rich repeat containing 3, Real Time...

Artikelnummer: VMPS-3567

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Lrrc3, mCG_5983, Leucine rich repeat containing 3
Anwendung: RNA quantification
43,00 €
Bewerten
Lrrc3, Rat leucine rich repeat containing 3, Real Time PCR Primer Set
Lrrc3, Rat leucine rich repeat containing 3, Real Time...

Artikelnummer: VRPS-3389

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Lrrc3, Leucine-rich repeat-containing protein 3
Anwendung: RNA quantification
52,00 €
Bewerten
Mouse ER Stress (UPR) Primer Library
Mouse ER Stress (UPR) Primer Library

Artikelnummer: MEPR-1

Contains 88 primer sets directed against ER stress genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM). Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction). Just add cDNA template and SYBR green master mix
Schlagworte: MEPR-I
Spezies-Reaktivität: mouse
854,00 €
Bewerten
Tnf, Mouse tumor necrosis factor, Real Time PCR Primer Set
Tnf, Mouse tumor necrosis factor, Real Time PCR Primer Set

Artikelnummer: VMPS-6717

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Tnf, Tnfa, TNF-a, Cachectin, TNF-alpha, Tumor necrosis factor, Tumor necrosis factor, soluble form, Tumor necrosis factor,...
Anwendung: RNA quantification
43,00 €
Bewerten
Il1B, Mouse interleukin 1 beta, Real Time PCR Primer Set
Il1B, Mouse interleukin 1 beta, Real Time PCR Primer Set

Artikelnummer: VMPS-3072

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Il1b, IL-1 beta, Interleukin-1 beta
Anwendung: RNA quantification
43,00 €
Bewerten
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