Anti-EZH2 (Enhancer of Zeste Homolog 2, Histone-lysine N-methyltransferase EZH2, ENX1, ENX-1, KMT6,

Anti-EZH2 (Enhancer of Zeste Homolog 2, Histone-lysine N-methyltransferase EZH2, ENX1, ENX-1, KMT6,
Item number Size Datasheet Manual SDS Delivery time Quantity Price
E9085-02D.20 20 µl - -

3 - 19 business days*

462.00€
E9085-02D.100 100 µl - -

3 - 19 business days*

834.00€
 
The polycomb group (PcG) proteins are involved in maintaining the silenced state of several... more
Product information "Anti-EZH2 (Enhancer of Zeste Homolog 2, Histone-lysine N-methyltransferase EZH2, ENX1, ENX-1, KMT6,"
The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large, protein family, contains four conserved regions including domain I, domain II, and a cysteine-richaa stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6). Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and ChIP. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:200, Immunofluorescence (frozen): 1:100, Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunohistochemistry (Paraffin): 1:50, Immunoprecipitation: 1:300, ChIP: 1:100, Flow Cytometry: 1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: EC=2.1.1.43, Anti-Enhancer of zeste homolog 2
Supplier: United States Biological
Supplier-Nr: E9085-02D

Properties

Application: IF, IHC, IP, WB
Antibody Type: Monoclonal
Clone: 11C1032(D2C9)
Conjugate: No
Host: Rabbit
Species reactivity: human, monkey, mouse, rat
Immunogen: Synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2.
Format: Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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