Platelet Derived Growth Factor-AA, human recombinant (rHuPDGF-AA-Yeast)

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Yeast

human

lyophilized

Cell culture

Biomol GmbH

97205.2

2 µg

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66 €

Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. (www.uniprot.org) Produced in Yeast, as a homodimeric, glycosilated, polypeptide chain containing 2 x 110 amino acids.

Cat-Nr:

97205.2

Menge:

2 µg

Preis:

66 €

UniProt ID: P04085 (Protein knowledgebase)

Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.

Introduction
PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.

Description
PDGF-AA Human Recombinant produced in Yeast is a homodimeric, glycosilated, polypeptide chain containing 2 x 110 amino acids and having a total molecular mass of 34 kDa.
PDGF-AA is purified by proprietary chromatographic techniques.

Source
Pichia Pastoris.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation
The protein was lyophilized with 20mM sodium phosphate buffer.

Solubility
It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability
Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity
Greater than 98.0% as determined by SDS-PAGE.

Biological Activity
The ED50 was found to be 1ng/ml corresponding to a Specific Activity of 1,000,000IU/mg calculated by the ability to stimulate the proliferation of mouse 3T3 fibroblasts (PNAS 94, 10205, 1997. Biochemistry, 1996, 35, 12077).

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