Anti-phospho-Ribosomal Protein S6 (Ser244, Ser247)

40S ribosomal protein S6 (also known as RPS6) is a ~31kD substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. Phosphorylation of RPS6 is rapamycin and wortmannin-sensitive as its activation is mediated by mTOR and PI3K pathways. Ribosomal protein S6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236?235?240?244?247, due to the positions of these amino acid residues on the alpha-helix. Hyperphosphorylation of ribosomal protein S6 stimulates protein synthesis that mediates progression through the cell cycle. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Recommended Positive Control: HeLa +/- TNF-a or anisomycin, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.