Antibody Anti-phospho-AKT (Ser473), clone 17B6.B11.A12.C3.G7.G12

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Antibody results 1

Anti-phospho-AKT (Ser473), clone 17B6.B11.A12.C3.G7.G12

17B6.B11 A12.C3 G7.G

Mouse
> sec. Ab

Mab

IgG1
kappa

human, mouse

expected: rat, chimpanzee

purified

ELISA
WB
IHC

Rockland

200-301-268

100 µg

299 €

Immunogen: synthetic peptide corresponding to residues surrounding Ser473 of human AKT1 protein

MW: 56 kD

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases).

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Cat #:

200-301-268

Size:

100 µg

Price:

299 €

UniProt ID: P31749 (Protein knowledgebase)

Antibody Conjugation Kits

Concentration:	1.0 mg/ml (by UV absorbance at 280 nm)
Physical State:	Liquid (sterile filtered)
Product Category:	Primary Antibodies, Phospho Specific Antibodies


Label:	Unconjugated


Host:	Mouse
Clone:	17B6.B11
Immunogen:	This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Gene:	AKT1
Reactivity:	human, mouse, rat, monkey
Buffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer:	None
Storage Conditions:	Store vial at -20° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
Synonyms:	RAC-PK-alpha, Protein kinase B, PKB, C-AKT
Preservative:	0.01% (w/v) Sodium Azide

Background:

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Application Notes:

This monoclonal antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.

Methods:

ELISA
Western Blot
Immunohistochemistry
Immunofluorescence Microscopy

Purity and Specificity:

This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.

Recommended Dilutions:

ELISA:	1:20,000
WB:	1:500 - 1:3,000
IHC:	20 µg/ml
IF:	1:500 - 1:3,000
OTHER:	User Optimized

Relevant Link(s):

P31749: http://www.uniprot.org/uniprot/P31749
NP_005154: http://www.ncbi.nlm.nih.gov/protein/62241011

General References:

Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910.

Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14.

Jones,P.F., Jakubowicz,T., Pitossi,F.J., Maurer,F. and Hemmings,B.A. (1991) Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc. Natl. Acad. Sci. U.S.A. 88 (10), 4171-4175.

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