Anti-p53 (Tumor Suppressor Protein, Oncogene Protein), clone 5F170

Applications:, Suitable for Immunohistochemistry (frozen and paraffin-embedded tissue, cytological material), Immunoblot, Immunoprecipitation, ELISA. Recommended Dilution: Immunohistochemistry: 1:500 with PBS, pH 7.4, when using the APAAP method, Pretreatment: No pretreatment required. Do not apply proteases. Incubation Time 1 hr at 37°C, extended with paraffin sections (overnight at 2-8°C). APAAP, PAP or streptavidin/ biotin method or application of signal amplification system is strongly recommended. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.