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Host:
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Rabbit
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Clone:
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Polyclonal
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Immunogen:
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This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.
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Gene:
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TUBA1B
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Reactivity:
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human, mouse, rat. chicken, bovine
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Buffer:
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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Stabilizer:
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None
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Storage Conditions:
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Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.
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Synonyms:
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Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous
Tubulin K-alpha-1, TUBA1B, tubulin loading control
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Preservative:
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0.01% (w/v) Sodium Azide
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Background:
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Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
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Application Notes:
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This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
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Methods:
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ELISA
Western Blot
Immunofluorescence Microscopy
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Purity and Specificity:
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This affinity purified antibody is directed against human alpha Tubulin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
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Recommended Dilutions:
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| ELISA: |
1:5,000 - 1:25,000 |
| WB: |
1:500 - 1:3,000 |
| IF: |
1:500 - 1:2,000 |
| OTHER: |
User Optimized |
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Relevant Link(s):
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P68363: http://www.uniprot.org/uniprot/P68363
NP_006000 : http://www.ncbi.nlm.nih.gov/protein/17986283
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General References:
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Hall,J.L. and Cowan,N.J. (1985) Structural features and restricted expression of a human alpha-tubulin gene. Nucleic Acids Res. 13 (1), 207-223.
Cowan,N.J., Dobner,P.R., Fuchs,E.V. and Cleveland,D.W. (1983) Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions. Mol. Cell. Biol. 3 (10), 1738-1745. |
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