Anti-Ribosomal Protein L8 (RPL8)

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications have not been tested. , Recommended Dilutions: Peptide ELISA Titer: 1:128,000, Western Blot: 0.01-0.03ug/ml ~28-30kD band observed in lysates of cell line HepG2 (calculated MW of 28.0kD according to NP_000964.1 and NP_150644.1), Immunohistochemistry: 2-4ug/ml In paraffin embedded Human Cerebral Cortex shows cytoplasm staining, including axons, of stallate and pyramidal cells., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.