Anti-Rabbit IgG HRP conjugated (Rabbit TrueBlot®), clone eB182

Anti-Rabbit IgG HRP conjugated (Rabbit TrueBlot®), clone eB182
Item number Size Datasheet Manual SDS Delivery time Quantity Price
18-8816-33 200 µl - -

approx. 1-3 business days

374.00€
 
Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting... more
Product information "Anti-Rabbit IgG HRP conjugated (Rabbit TrueBlot®), clone eB182"
Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional HRP anti-rabbit IgG blotting reagent with Rabbit IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.
Keywords: TrueBlot®, IP/WB
Supplier: Rockland Immunochemicals
Supplier-Nr: 18-8816-33

Properties

Application: WB
Antibody Type: Monoclonal
Clone: eB182
Conjugate: HRP
Host: Mouse
Species reactivity: rabbit

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Anti-Rabbit IgG HRP conjugated (Rabbit TrueBlot®), clone eB182"
16 May 2019

Highly recommended

Amazing! Close to undetectable levels of IgG heavy chain in the IP samples. Can only be seen after extremely long exposures. Highly recommended!

Write a review
or to review a product.
Viewed