Bemerkungen:The RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II core complex. RNA polymerase II holoenzyme functions to transcribe DNA into messenger RNA in eukaryotes. RPB1 forms the DNA binding domain of the polymerase that forms a cleft that grabs the incoming DNA template to translocate the holoenzyme across the RNA-DNA hybrid. Alternate identifiers of RPB1 include DNA-directed RNA polymerase II subunit RPB1, DNA directed RNA polymerse II subunit A, DNA-directed RNA polymerase II largest subunit, POLR2A, RPO2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220, and MGC75453.
RNA Polymerase II Antibody
Rabbit anti-RNA Polymerase II Antibody, Affinity Purified
Detection of Human and Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 µg for WB), HeLa (H; 50 µg), Jurkat (J; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A (lot A300-653A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human RNA Polymerase II by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from 293T cells. Antibody: Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A (lot A300-653A-3) used for WB at 1 µg/ml and used for IP at 6 µg/mg lysate. RNA Polymerase II was also immunoprecipitated by a previous lot (lot A300-653A-2) of this antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.
This antibody is qualified for the Proximity Ligation Assay (PLA). PLA uses paired antibodies, so please refer to the PLA page of our website for specific imaging obtained by pairing this antibody with other qualified antibodies. The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
Gene and Protein Information
Rat, Zebrafish, X. tropicalis, Bovine, Dog, Rabbit, Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Castor bean, Honeybee, Lyre-leaved rock-cress_81972, Eukaryota, Naked mole rat, African elephant, Caenorhabditis elegans, Amoeba_5755, Barrel medic, Grape, Mouse-ear cress, Japanese pufferfish, Northern white-cheeked gibbon, Little brown bat, Gray short-tailed opossum, African rice, Bakers yeast_559292, Fission yeast, Microsporidian parasite_284813, Moss_145481, Soybean, Slime mold_1054147, Rice, Slime mold_44689, Sorghum_4558, Thirteen-lined ground squirrel, Small-eared galago, Leafcutter ant, Spikemoss_88036, Spotted green pufferfish, Potato, Purple false brome, Purple sea urchin, Rhodophyta, Chinese hamster, Brassicaceae, Yeast_284811, West Indian ocean coelacanth, African malaria mosquito, Nematode worm_135651, Green anole, Nile tilapia, White campion_37657, Pacific transparent sea squirt, Panamanian leafcutter ant, Two-rowed barley, White-tufted-ear marmoset, Tasmanian devil, Yeast_294746, Caenorhabditis briggsae less...
Garriga J., CDK9 inhibition strategy defines distinct sets of target genes BMC Res Notes. 2014 May 16;7:301. WB
Berlivet S, Clustering of Tissue-Specific Sub-TADs Accompanies the Regulation of HoxA Genes in Developing Limbs. PLoS Genet. 2013 Dec;9(12):e1004018. ChIP
Lovén J, Selective inhibition of tumor oncogenes by disruption of super-enhancers. Cell. 2013 Apr 11;153(2):320-34. ChIP
Wu Y et al., Cyclin-dependent kinase 7/9 inhibitor SNS-032 abrogates FIP1-like-1 platelet-derived growth factor receptor ? and bcr-abl oncogene addiction in malignant hematologic cells. Clin Cancer Res. 2012 Apr 1;18(7):1966-78. WB
Schmutz I et al., Protein phosphatase 1 (PP1) is a post-translational regulator of the mammalian circadian clock. PLoS One. 2011;6(6):e21325. WB
Pakala SB et al., TGF-?1 signaling targets metastasis-associated protein 1, a new effector in epithelial cells. Oncogene. 2011 May 12;30(19):2230-41. WB, ChIP
Ghanta KS et al., MTA1 coregulation of transglutaminase 2 expression and function during inflammatory response. J Biol Chem. 2011 Mar 4;286(9):7132-8. ChIP, WB
Lucio-Eterovic AK et al., Role for the nuclear receptor-binding SET domain protein 1 (NSD1) methyltransferase in coordinating lysine 36 methylation at histone 3 with RNA polymerase II function. Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16952-7. WB
Pakala SB et al., MTA1 coregulator regulates LPS response via MyD88-dependent signaling. J Biol Chem. 2010 Oct 22;285(43):32787-92. ChIP, WB
Pakala SB et al., Regulation of NF-kappaB circuitry by a component of the nucleosome remodeling and deacetylase complex controls inflammatory response homeostasis. J Biol Chem. 2010 Jul 30;285(31):23590-7. WB, IP, ChIP
Schmutz I et al., The mammalian clock component PERIOD2 coordinates circadian output by interaction with nuclear receptors. Genes Dev. 2010 Feb 15;24(4):345-57. WB
Glover-Cutter K et al., TFIIH-associated Cdk7 kinase functions in phosphorylation of C-terminal domain Ser7 residues, promoter-proximal pausing, and termination by RNA polymerase II. Mol Cell Biol. 2009 Oct;29(20):5455-64. WB