Anti-Proteasome 19S, Subunit S5A (PSMD4)

S5a(PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinylated-protein targeting and degradation. S5a contains two polyubiquitin binding motifs (UIM) which bind multiubiquitin chains by hydrophobic interaction. In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of protein directly and mediates their targeting to the proteasome for degradation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.