Anti-Phosphothreonine, clone 3i26

Much of the dynamic behavior of cellular proteins, including the regulation of molecular interactions (1), subcellular localization (2) and transcription (3) are controlled by a variety of posttranslational modifications (4). Antibodies specific for these posttranslational modifications are invaluable tools in the quest to understand normal and pathogenic molecular and cellular behavior. General protein modification antibodies are designed to react with modified amino acid residues (e.g., phosphothreonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs. Protein kinases are among the most abundant eukaryotic regulatory proteins, over 500 separate kinase genes are encoded in mammalian genomes (5). In spite of the importance of kinases in eukaryotic biology, relatively few of their physiological targets are known. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Immunoprecipitation: 1:50, ELISA: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.