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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
I8434-50C.100 | 100 tests | - | - |
3 - 19 business days* |
901.00€
|
The biological activities of IL-12 are mediated through a high-affinity receptor complex composed... more
Product information "Anti-Interleukin 12 receptor, Beta 2 (IL-12-Rb2 , beta 2 IL-12R-beta-2, IL-12 receptor beta-2, Inte"
The biological activities of IL-12 are mediated through a high-affinity receptor complex composed of the IL-12 receptor beta 1 (IL-12 Rbeta1) and IL-12 receptor beta2 (IL-12 Rbeta2) subunits. IL-12 Rbeta2 is a 130kD protein that interacts with p35 and is associated with Jak2. The expression of IL-12 Rbeta2 is absent on nai?ve T cells. Activation of T cells via TCR upregulates IL-12 Rbeta2 expression on human Th1, but not Th2 cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. , Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction., 2. After blocking, add 10ul of I8434-50C to up to 1x10e6 cells and incubate for 30 minutes at RT., 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer., 4. Resuspend the cells in Flow Cytometry Staining Buffer for analysis by flow cytometry. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Keywords: | Anti-IL12RB2, Anti-IL-12RB2, Anti-IL-12R subunit beta-2, Anti-IL-12 receptor subunit beta-2, Anti-Interleukin-12 receptor subunit beta-2 |
Supplier: | United States Biological |
Supplier-Nr: | I8434-50C |
Properties
Application: | FC |
Antibody Type: | Monoclonal |
Clone: | 9H142 |
Conjugate: | APC |
Host: | Mouse |
Species reactivity: | human, mouse |
Immunogen: | NS0-derived, recombinant human Interleukin 12 Receptor beta 2, aa 24 - 622, Q99665, extracellular domain. |
Format: | Affinity Purified |
Database Information
KEGG ID : | K05064 | Matching products |
UniProt ID : | Q99665 | Matching products |
Gene ID | GeneID 3595 | Matching products |
Handling & Safety
Storage: | +4°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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