158 products were found matching "H5110-15X"!

Sequence: SGRGKGGKGLGKGGAKRHRKVLR-GSGSK-biotin corresponding to amino acids 2-24 of Histone H4 followed by a GSGS linker and biotinylated lysine. Applications: Suitable for use in HAT assay. Other applications not tested. Recommended Dilution: HAT Assay: 1ug of active PCAF acetylated this peptide 140-fold over...

Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders (1). In one study, the predominant responses to histones in SLE sera were...

Kit Components:, H5110-15J1: Histone H4, acetylated (Lys5) 1x25ul , H5110-15J2: Histone H4, acetylated (Lys8) 1x20ul, H5110-15J3: Histone H4, acetylated (Lys12) 1x25ug, H5110-15J4: Histone H4, acetylated (Lys16) 1x20ul, Quality Control Testing Immunoblot Analysis: Histone H4, acetylated antibodies detected increased...

Sequence: SGRGKGG[AcK]GLGKGGAKRHR-C, which corresponds to the N terminal sequence of histone H4 acetylated at position 8 plus a C terminal cysteine. Application(s): May be used to measure histone acetyltransferase (HAT) activity. May be acetylated by HAT on lysines at position 5, 12 and 16. May be used to determine...

Substrate for HAT activity, Sequence: SGRGKGGKGLGKGGAKRHR-C. Residues 2-20 corresponding to the N-terminal sequence of Histone H4. Application(s): The peptide can be used to measure histone acetyltransferase (HAT) activity. When used with peptides of identical sequence but differing sites of acetylation, it can be...

Sequence: AGGACKGGACKGMGACKVGAACKRHSC, Residues 2-19 (Ac K5-K8-K12-K16), corresponding to the N-terminal sequence of Tetrahymena Histone H4. The four lysine residues are acetylated. The acetylation of lysine residues in core histones is thought to play a role in the activation of gene transcription. The...

Sequence: SGRGKGGKGLGKGGA-Ac-KRHRC. Corresponds to the N-terminal sequence of Histone H4 acetylated at position 16. Applications: HAT Assay: Use 1ug of peptide per assay. A previous lot was tested by using 50ng of GCN5 to acetylate the peptide. Yeast GCN5 does not effectively acetylate this peptide since the...

Applications: , Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ul detected Histone H4 in acid extracts of HeLa cells treated with or without 5mM sodium butyrate. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at...

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker...

Applications:, Western Blotting: 1:2000 dilution detects acetylated histone H4 in Tetrahymena macronuclei and acid extracted proteins from HeLa cells treated with 5mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4., Chromatin...

Applications: , Suitable for use in Western Blot and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 dilution of this lot detected acetyl-Histone H4 (Lys5) in acid extracts from sodium butyrate treated HeLa cells , ChIP: An independent laboratory has shown that this antibody...

Applications:, Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:500, ELISA: 1:1000, Western Blot: 1:500-1:1000, Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml, Optimal dilutions to...